基于磁性酶联免疫法的食品中大肠杆菌O157:H7的化学发光检测
[Chemiluminescent detection of E. coli O157:H7 in food based on magnetic enzyme-linked immuno method].
作者信息
Guo Xiao-ying, Wang Yong-ning, Gu Lin-gang, Liu He-ping, He Yan-feng, Lu Zu-hong
机构信息
State Key Laboratory for Bioelectronics, Southeast University, Nanjing 210096, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 May;22(3):395-8.
AIM
To establish a new chemiluminescent assay with high sensitivity for detecting E. coli O157:H7 in food.
METHODS
The assay was established based on the alkaline phosphatase (ALP) labeled anti-E. coli O157:H7 antibody, which reacted with an excellent chemiluminescent reagent, 3(2' spiroadamantane)4 methoxy 4(3''phosphoryloxy) phenyl 1,2 dioxetane (AMPPD) buffer solution and emitted the photons.
RESULTS
The sensitivity of chemiluminescence assay was 850, and the linear range was 1000-50,000. The intra- and interassay CV values (CVs) were respectively below 15% and 20%. The correlation coefficient was 0.9807 by comparing with the conventional counting method.
CONCLUSION
The chemiluminescent immunoassay is more accurate than other methods in the detection of concentration of E. coli O157:H7 in samples.
目的
建立一种高灵敏度的化学发光分析法，用于检测食品中的大肠杆菌O157:H7。
方法
该分析法基于碱性磷酸酶（ALP）标记的抗大肠杆菌O157:H7抗体，其与一种优良的化学发光试剂3-(2'-螺金刚烷)-4-甲氧基-4-(3''-磷酰氧基)苯基-1,2-二氧杂环丁烷（AMPPD）缓冲溶液反应并发射光子。
结果
化学发光分析法的灵敏度为850，线性范围为1000 - 50000。批内和批间变异系数（CV）分别低于15%和20%。与传统计数方法相比，相关系数为0.9807。
结论
在检测样品中大肠杆菌O157:H7的浓度时，化学发光免疫分析法比其他方法更准确。
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