依赖巯基辅酶A的分枝杆菌对氧化应激的反应。

Mycothiol-dependent mycobacterial response to oxidative stress.

作者信息

Ung Korine S E, Av-Gay Yossef

机构信息

Division of Infectious Diseases, Department of Medicine, The University of British Columbia, D-452 Heather Pavilion East, 2733 Heather Street, Vancouver, BC, Canada V5Z 3J5.

出版信息

FEBS Lett. 2006 May 15;580(11):2712-6. doi: 10.1016/j.febslet.2006.04.026. Epub 2006 Apr 21.

DOI:10.1016/j.febslet.2006.04.026

PMID:16643903

Abstract

The effect of exogenous oxidative stress on mycothiol (MSH) levels and redox balance was investigated in mycobacteria. Both the thiol-specific oxidant diamide and hydrogen peroxide induced up to 75% depletion of MSH to form the disulfide form, mycothione (MSSM), in Mycobacterium bovis BCG. In comparison, Mycobacterium smegmatis, a saprophytic mycobacterium, displays a greater tolerance towards these oxidants, reflected by the lack of fluxes in MSH levels and redox ratios upon oxidative stress treatments. The basal ratio of MSH to MSSM was established to be 50:1 in M. bovis BCG and 200:1 in M. smegmatis.

摘要

研究了外源性氧化应激对分枝杆菌中麦角硫因（MSH）水平和氧化还原平衡的影响。硫醇特异性氧化剂二酰胺和过氧化氢均可使牛分枝杆菌卡介苗（Mycobacterium bovis BCG）中的MSH耗竭高达75%，形成二硫键形式的麦角硫因二硫化物（MSSM）。相比之下，腐生分枝杆菌耻垢分枝杆菌（Mycobacterium smegmatis）对这些氧化剂表现出更高的耐受性，这体现在氧化应激处理后MSH水平和氧化还原比率没有变化。牛分枝杆菌卡介苗中MSH与MSSM的基础比率为50:1，耻垢分枝杆菌中为200:1。