伤寒沙门氏菌Vi抗原B区域的分子克隆

Molecular cloning of the ViaB region of Salmonella typhi.

作者信息

Hashimoto Y, Ezaki T, Li N, Yamamoto H

机构信息

Department of Microbiology, Gifu University School of Medicine, Japan.

出版信息

FEMS Microbiol Lett. 1991 Dec 15;69(1):53-6. doi: 10.1016/0378-1097(91)90645-q.

DOI:10.1016/0378-1097(91)90645-q

PMID:1664401

Abstract

The ViaB region required for Vi antigen production in Salmonella typhi was cloned. The plasmid pGBM124 containing a 14-kb S. typhi chromosomal DNA fragment conferred the ability to produce Vi antigen on Escherichia coli HB101 and ViaB-deleted S. typhi GIFU10007-3. Tn5 insertion analysis showed that the 14-kb DNA was split into three regions. Region 1 and region 2 are involved in the biosynthesis of Vi polysaccharide. Region 3 is involved in translocation of the Vi polysaccharide to the cell surface. Southern blot hybridization showed that regions 2 and 3 but not region 1, were considerably homologous to the DNA of Vi-positive Citrobacter freundii.

摘要

伤寒沙门氏菌中Vi抗原产生所需的ViaB区域被克隆。含有14kb伤寒沙门氏菌染色体DNA片段的质粒pGBM124赋予大肠杆菌HB101和缺失ViaB的伤寒沙门氏菌GIFU10007-3产生Vi抗原的能力。Tn5插入分析表明，14kb的DNA被分成三个区域。区域1和区域2参与Vi多糖的生物合成。区域3参与Vi多糖向细胞表面的转运。Southern印迹杂交表明，区域2和区域3而非区域1与Vi阳性弗氏柠檬酸杆菌的DNA有相当高的同源性。