Arriaga Sonia, Muñoz Raúl, Hernández Sergio, Guieysse Benoit, Revah Sergio
Departamento de Ingeniería de Procesos e Hidraúlica, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlíxco No. 186, Col. Vicentina, P.O. Box 55-534, 09340, Mexico City, Mexico.
Environ Sci Technol. 2006 Apr 1;40(7):2390-5. doi: 10.1021/es051512m.
Biofiltration of hydrophobic volatile pollutants is intrinsically limited by poor transfer of the pollutants from the gaseous to the liquid biotic phase, where biodegradation occurs. This study was conducted to evaluate the potential of silicone oil for enhancing the transport and subsequent biodegradation of hexane by the fungus Fusarium solani in various bioreactor configurations. Silicone oil was first selected among various solvents for its biocompatibility, nonbiodegradability, and good partitioning properties toward hexane. In batch tests, the use of silicone oil improved hexane specific biodegradation by approximately 60%. Subsequent biodegradation experiments were conducted in stirred-tank (1.5 L) and packed-bed (2.5 L) bioreactors fed with a constant gaseous hexane load of 180 g x m(-3)(reactor) x h(-1) and operated for 12 and 40 days, respectively. In the stirred reactors, the maximum hexane elimination capacity (EC) increased from 50 g x m(-3)(reactor) x h(-1) (removal efficiency, RE of 28%) in the control not supplied with silicone oil to 120 g x m(-3)(reactor) x h(-1) in the biphasic system (67% RE). In the packed-bed bioreactors, the maximum EC ranged from 110 (50% RE) to 180 g x m(-3)(reactor) x h(-1) (> 90% RE) in the control and two-liquid-phase systems, respectively. These results represent, to the best of our knowledge, the first reported case of fungi use in a two-liquid-phase bioreactor and the highest hexane removal capacities so far reported in biofilters.
疏水性挥发性污染物的生物过滤本质上受到污染物从气相到发生生物降解的液相生物相转移不良的限制。本研究旨在评估硅油在各种生物反应器配置中增强真菌茄病镰刀菌对己烷的传输及后续生物降解的潜力。硅油因其生物相容性、不可生物降解性以及对己烷良好的分配特性，首先从各种溶剂中被挑选出来。在分批试验中，使用硅油使己烷的比生物降解率提高了约60%。随后在搅拌罐（1.5升）和填充床（2.5升）生物反应器中进行生物降解实验，分别以180克·立方米⁻³（反应器）·小时⁻¹的恒定气态己烷负荷进料，运行12天和40天。在搅拌反应器中，最大己烷去除能力（EC）从未添加硅油的对照中的50克·立方米⁻³（反应器）·小时⁻¹（去除效率，RE为28%）增加到双相系统中的120克·立方米⁻³（反应器）·小时⁻¹（RE为67%）。在填充床生物反应器中，对照和双液相系统中的最大EC分别为110（RE为50%）至180克·立方米⁻³（反应器）·小时⁻¹（>90% RE）。据我们所知，这些结果代表了首次报道的真菌在双液相生物反应器中的应用案例，也是迄今为止生物滤池中报道的最高己烷去除能力。
