Frostholm A, Zdilar D, Chang A, Rotter A
Department of Pharmacology, Ohio State University, Columbus 43210.
Brain Res Dev Brain Res. 1991 Dec 17;64(1-2):121-8. doi: 10.1016/0165-3806(91)90215-5.
A [35S]cRNA probe was used for the visualization of GABAA/benzodiazepine (GABAA/BZ) receptor alpha 1 subunit mRNA in developing reeler mutant mouse cerebellum. A clear hybridization signal was observed throughout the malformed reeler cerebellum from birth. Labeling was associated with Purkinje cell bodies located in three subcortical masses. Additional labeled Purkinje cells were observed within the granule cell layer and at their normal position at the interface between the molecular and granule cell layers. All reeler Purkinje cells had comparable levels of grain density, regardless of their location within the cerebellar cortex. These results indicate that Purkinje cell malpositioning, and the resulting absence of a major complement of afferents throughout development, does not impair the expression of mRNA coding for the alpha 1 subunit of the GABAA/BZ receptor.
使用[35S] cRNA探针来观察发育中的Reeler突变小鼠小脑内GABAA/苯二氮䓬（GABAA/BZ）受体α1亚基mRNA。从出生起，在畸形的Reeler小脑中就观察到清晰的杂交信号。标记与位于三个皮质下团块中的浦肯野细胞体相关。在颗粒细胞层内以及分子层与颗粒细胞层界面的正常位置也观察到了额外的标记浦肯野细胞。所有Reeler浦肯野细胞，无论其在小脑皮质内的位置如何，都具有相当的颗粒密度水平。这些结果表明，浦肯野细胞位置异常以及在整个发育过程中由此导致的主要传入神经补充缺失，并不损害编码GABAA/BZ受体α1亚基的mRNA的表达。
