Molenaar W M, de Leij L, Trojanowski J Q
Department of Pathology, University of Pennsylvania, Philadelphia.
Acta Neuropathol. 1991;83(1):46-54. doi: 10.1007/BF00294429.
The current study describes the presence of neuroendocrine antigens of peripheral and central neural tumors using eight monoclonal antibodies raised to small cell lung carcinoma (SCLC), which recognize "neural/neuroendocrine" or "neural" antigens, as defined by their reaction pattern in normal tissues and tumors. At least five of them recognize different epitopes of the neural cell adhesion molecule (N-CAM). It was found that all of 12 neuroblastomas, 2 ganglioneuroblastomas and 4 ganglioneuromas as well as 23 central primitive neuroectodermal tumors, 13 astrocytomas and 4 ependymomas share "neural/neuroendocrine" antigens (as defined by the anti-N-CAM antibodies Moc-1, -21, -32, -52 and -191) with SCLC. The "neural/neuroendocrine" antigen defined by Moc-171 was also found in all peripheral tumors, but only in further differentiated central tumors. Non-N-CAM related "neural" antigens (as defined by Moc-51 and -172) were found only in better-differentiated peripheral and central tumors, but they could be demonstrated in all three medulloblastoma cell lines studied. In addition, the antigen defined by Moc-51 was demonstrated in an immunoblot of a neuroblastoma cell line. Antibodies recognizing "epithelial" antigens of SCLC and other epithelia and their tumors (Moc-31 and -181) were non-reactive. It was concluded that these findings give further support for a relation between neural and neuroendocrine tumors and that some of the antibodies may be useful for the detection of differentiation in neural tumors. Antibodies with an "epithelial" recognition pattern may serve to distinguish neural from neuroendocrine tumors.
本研究利用针对小细胞肺癌（SCLC）产生的8种单克隆抗体，描述外周和中枢神经肿瘤神经内分泌抗原的存在情况。这些抗体根据其在正常组织和肿瘤中的反应模式，识别“神经/神经内分泌”或“神经”抗原。其中至少5种识别神经细胞黏附分子（N-CAM）的不同表位。研究发现，12例神经母细胞瘤、2例神经节神经母细胞瘤、4例神经节瘤以及23例中枢原始神经外胚层肿瘤、13例星形细胞瘤和4例室管膜瘤，均与SCLC共享“神经/神经内分泌”抗原（由抗N-CAM抗体Moc-1、-21、-32、-52和-191定义）。由Moc-171定义的“神经/神经内分泌”抗原在所有外周肿瘤中也有发现，但仅在进一步分化的中枢肿瘤中存在。非N-CAM相关的“神经”抗原（由Moc-51和-172定义）仅在分化较好的外周和中枢肿瘤中发现，但在所有研究的3种髓母细胞瘤细胞系中均可检测到。此外，由Moc-51定义的抗原在神经母细胞瘤细胞系的免疫印迹中得到证实。识别SCLC和其他上皮及其肿瘤“上皮”抗原的抗体（Moc-31和-181）无反应。研究得出结论，这些发现进一步支持了神经肿瘤与神经内分泌肿瘤之间的关系，并且一些抗体可能有助于检测神经肿瘤的分化。具有“上皮”识别模式的抗体可用于区分神经肿瘤和神经内分泌肿瘤。
