[乙醇对豚鼠离体壁细胞酸分泌的影响]
[Effects of ethanol on acid secretion by isolated parietal cells from guinea pig].
作者信息
Niki S, Nakamura K, Rokutan K, Saitoh T, Aoike A, Kawai K
机构信息
Department of Preventive Medicine, Kyoto Prefectural University of Medicine.
出版信息
Nihon Shokakibyo Gakkai Zasshi. 1991 Sep;88(9):2094-100.
We studied the effects of ethanol (0.1-10%) on acid secretion of parietal cell-rich fractions isolated from guinea pig gastric mucosa. Ethanol (0.1-3%) increased histamine-stimulated cAMP content, while over 1% ethanol decreased histamine-stimulated acid secretion. H+, K(+)-ATPase activity in microsomal fraction also decreased after treatment with 3% ethanol. Thus, ethanol may disturb the signalling process from cAMP to H+, K(+)-ATPase. On the other hand, carbachol-stimulated acid secretion was more sensitive to ethanol than that with histamine, and 0.1% ethanol suppressed the acid secretion. This effect was well correlated with the extent of the ethanol-induced increase of [Ca2+]i and with the attenuation of [Ca2+]i response following carbachol stimulation. The calcium response may be a primary target against ethanol in carbachol-dependent process. In conclusion, low-dose ethanol have multi-effects on these critical intermediary steps in acid secretion.
我们研究了乙醇（0.1 - 10%）对从豚鼠胃黏膜分离出的富含壁细胞部分的胃酸分泌的影响。乙醇（0.1 - 3%）增加了组胺刺激的环磷酸腺苷（cAMP）含量，而超过1%的乙醇则降低了组胺刺激的胃酸分泌。用3%乙醇处理后，微粒体部分的H⁺，K⁺ - ATP酶活性也降低。因此，乙醇可能会干扰从cAMP到H⁺，K⁺ - ATP酶的信号传导过程。另一方面，卡巴胆碱刺激的胃酸分泌比组胺刺激的胃酸分泌对乙醇更敏感，0.1%的乙醇就可抑制胃酸分泌。这种作用与乙醇诱导的细胞内钙离子浓度（[Ca²⁺]i）升高程度以及卡巴胆碱刺激后[Ca²⁺]i反应的减弱密切相关。在卡巴胆碱依赖性过程中，钙反应可能是乙醇作用的主要靶点。总之，低剂量乙醇对胃酸分泌的这些关键中间步骤具有多种影响。
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