Kasinsky H E, Shichi H, Hackett D P
Department of Biochemistry, University of California, Berkeley, California.
Plant Physiol. 1966 May;41(5):739-48. doi: 10.1104/pp.41.5.739.
Efforts have been made to solubilize cytochrome components from particulate fractions of etiolated mung bean seedlings. Low temperature spectrophotometry reveals that the cytochrome composition of mitochondria isolated from whole seedlings is the same as that reported by Bonner for mung bean hypocotyls. On the basis of the identity in position of the alpha-bands in low temperature difference spectra for mitochondria, for a partially purified haemoprotein from mitochondria, and for purified cytochrome b-555, it is suggested that cytochrome b-555 is an intrinsic component of mung bean mitochondria. Difference spectra show that both the mitochondrial and microsomal fractions contain at least 2 b-type cytochromes. Cytochrome b-555 is almost certainly present in the microsomes, since the low temperature difference spectrum for the cytochrome is identical with the spectrum for this particulate fraction.By freezing and thawing mung bean mitochondria in 4% cholate and centrifuging, cytochrome oxidase activity can be concentrated in the supernatant fraction, although it is not completely solubilized. The oxidase is inhibited by high concentrations of cytochrome c. A particle-bound cytochrome c can be obtained from mitochondria by digestion with snake venom. However, the autoxidizability of the preparation indicates that the cytochrome has been solubilized in a modified form. A CO-binding pigment can be obtained from mung bean microsomes by digestion with snake venom.
人们已致力于从黄化绿豆幼苗的颗粒部分中溶解细胞色素成分。低温分光光度法显示，从整株幼苗中分离出的线粒体的细胞色素组成与邦纳报道的绿豆下胚轴的细胞色素组成相同。基于线粒体、线粒体部分纯化的血红素蛋白以及纯化的细胞色素b - 555在低温差光谱中α带位置的一致性，表明细胞色素b - 555是绿豆线粒体的固有成分。差光谱表明线粒体和微粒体部分都至少含有2种b型细胞色素。细胞色素b - 555几乎肯定存在于微粒体中，因为该细胞色素的低温差光谱与该颗粒部分的光谱相同。通过在4%胆酸盐中冷冻和解冻绿豆线粒体并离心，细胞色素氧化酶活性可集中在上清液部分，尽管它并未完全溶解。该氧化酶受到高浓度细胞色素c的抑制。通过用蛇毒消化可从线粒体中获得一种颗粒结合的细胞色素c。然而，该制剂的自动氧化能力表明细胞色素已以一种修饰形式溶解。通过用蛇毒消化可从绿豆微粒体中获得一种与一氧化碳结合的色素。
