离体叶片在培养过程中氨基酸含量的变化。III. 糖在萎蔫叶片脯氨酸积累中的作用。
Changes in Amino Acid Content of Excised Leaves During Incubation. III. Role of Sugar in the Accumulation of Proline in Wilted Leaves.
机构信息
U. S. Plant, Soil and Nutrition Laboratory, United States Department of Agriculture, Tower Road, Ithaca, N. Y. 14850.
出版信息
Plant Physiol. 1966 Dec;41(10):1585-90. doi: 10.1104/pp.41.10.1585.
A previously reported accumulation of proline in wilted turnip leaves has been observed in 6 additional species representing 5 different families. The results of experiments on the conditions affecting the behavior of proline in wilted leaves were interpreted to mean that the supply and metabolism of available carbohydrate was essential for proline accumulation.The role of sugar in proline accumulation was determined by incubating wilted leaves with different levels of carbohydrate (produced either by variations in conditions of preillumination or by infiltration with sucrose). The results showed that proline accumulation was greater and most prolonged in wilted leaves with higher sugar and starch contents. No proline accumulation was observed in unwilted leaves. Also some inhibitors of glycolysis and of the tricarboxylic acid cycle prevented proline accumulation in wilted leaves.Based on these results, the interpretation of the role of sugar in proline accumulation is that the oxidation of sugars furnishes alpha-ketoglutarate and NAD(P)H for proline synthesis in wilted leaves.
先前在萎蔫的萝卜叶片中发现脯氨酸积累的现象，在另外 5 个不同科的 6 个种中也观察到了。对影响萎蔫叶片中脯氨酸行为的条件进行实验，结果表明，可利用碳水化合物的供应和代谢对脯氨酸积累是必需的。糖在脯氨酸积累中的作用是通过用不同水平的碳水化合物（通过预照光条件的变化或用蔗糖渗透产生）来孵育萎蔫叶片来确定的。结果表明，在具有较高糖和淀粉含量的萎蔫叶片中，脯氨酸积累更多且时间更长。在未萎蔫的叶片中未观察到脯氨酸积累。此外，一些糖酵解和三羧酸循环的抑制剂也可防止萎蔫叶片中脯氨酸的积累。基于这些结果，对糖在脯氨酸积累中作用的解释是，糖的氧化为萎蔫叶片中脯氨酸的合成提供了α-酮戊二酸和 NAD(P)H。
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