大豆（Glycine max Merr.）根瘤中固氮酶的纯化及某些性质
Purification and Some Properties of the Nitrogenase From Soybean (Glycine max Merr.) Nodules.
作者信息
Klucas R V, Koch B, Russell S A, Evans H J
机构信息
Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331.
出版信息
Plant Physiol. 1968 Dec;43(12):1906-12. doi: 10.1104/pp.43.12.1906.
Abstract
The nitrogenase system in cell-free extracts of soybean nodule bacteroids was fractionated into 2 components by use of protamine sulfate or polypropylene glycol precipitation followed by chromatography on DEAE-cellulose. Iron and molybdenum were concentrated in 1 fraction and iron in the other. Combination of fractions resulted in a striking stimulation in activity relative to the activity of individual fractions. The effect of different proportions of the 2 fractions on specific activities was studied. The ratios of the rates of reduction of acetylene and N(2) by extracts or fractions of different purities were relatively constant. Extracts or fractions retained most of their nitrogenase activities when stored in liquid N(2).
摘要
通过使用硫酸鱼精蛋白或聚丙二醇沉淀，随后在DEAE - 纤维素上进行色谱分离，将大豆根瘤类菌体无细胞提取物中的固氮酶系统分成两个组分。铁和钼集中在一个组分中，而另一个组分中只有铁。组分的组合相对于各个组分的活性导致活性显著增强。研究了两种组分不同比例对比活性的影响。不同纯度的提取物或组分对乙炔和N₂的还原速率之比相对恒定。提取物或组分保存在液氮中时，大部分固氮酶活性得以保留。
相似文献
1
Purification and Some Properties of the Nitrogenase From Soybean (Glycine max Merr.) Nodules.大豆（Glycine max Merr.）根瘤中固氮酶的纯化及某些性质
Plant Physiol. 1968 Dec;43(12):1906-12. doi: 10.1104/pp.43.12.1906.
2
Purification and some properties of a non-haem iron protein from the bacteroids of soya-bean (Glycine max Merr) nodules.来自大豆（Glycine max Merr）根瘤类菌体的一种非血红素铁蛋白的纯化及某些性质
Biochem J. 1970 Aug;118(5):773-81. doi: 10.1042/bj1180773.
3
Poly-beta-hydroxybutyrate Utilization by Soybean (Glycine max Merr.) Nodules and Assessment of Its Role in Maintenance of Nitrogenase Activity.大豆（Glycine max Merr.）根瘤对聚-β-羟基丁酸的利用及其在维持固氮酶活性中的作用评估
Plant Physiol. 1971 Jun;47(6):750-5. doi: 10.1104/pp.47.6.750.
4
Effect of nitrate on the organic Acid and amino Acid composition of legume nodules.硝酸盐对豆科植物根瘤有机酸和氨基酸组成的影响。
Plant Physiol. 1987 Nov;85(3):774-9. doi: 10.1104/pp.85.3.774.
5
Nitrate and Nitrite Reduction in Relation to Nitrogenase Activity in Soybean Nodules and Rhizobium japonicum Bacteroids.大豆根瘤和日本根瘤菌类菌体中硝酸盐和亚硝酸盐还原与固氮酶活性的关系
Plant Physiol. 1983 Apr;71(4):731-5. doi: 10.1104/pp.71.4.731.
6
Composition and Distribution of Adenylates in Soybean (Glycine max L.) Nodule Tissue.大豆（Glycine max L.）根瘤组织中腺苷酸的组成与分布
Plant Physiol. 1994 Jan;104(1):217-225. doi: 10.1104/pp.104.1.217.
7
The nitrogenase proteins of Rhizobium meliloti: purification and properties of the MoFe and Fe components.苜蓿根瘤菌的固氮酶蛋白：钼铁蛋白和铁蛋白组分的纯化及特性
Biochim Biophys Acta. 1993 Apr 21;1163(1):31-41. doi: 10.1016/0167-4838(93)90275-v.
8
Proteolytic Activity in Soybean Root Nodules : Activity in Host Cell Cytosol and Bacteroids throughout Physiological Development and Senescence.大豆根瘤中的蛋白水解活性：在生理发育和衰老过程中宿主细胞质和类菌体中的活性。
Plant Physiol. 1983 Apr;71(4):797-802. doi: 10.1104/pp.71.4.797.
9
Studies on soybean nodule senescence.关于大豆结瘤衰老的研究。
Plant Physiol. 1974 Oct;54(4):612-6. doi: 10.1104/pp.54.4.612.
10
Purification and characterization of the molybdenum-iron protein component of nitrogenase from soybean nodule bacteroids.大豆根瘤类菌体中固氮酶钼铁蛋白组分的纯化与特性分析
J Biol Chem. 1974 Jan 25;249(2):500-8.
引用本文的文献
1
Poly-beta-hydroxybutyrate Utilization by Soybean (Glycine max Merr.) Nodules and Assessment of Its Role in Maintenance of Nitrogenase Activity.大豆（Glycine max Merr.）根瘤对聚-β-羟基丁酸的利用及其在维持固氮酶活性中的作用评估
Plant Physiol. 1971 Jun;47(6):750-5. doi: 10.1104/pp.47.6.750.
2
Symbiotic effectiveness and n(2) fixation in nodulated soybean.根瘤大豆中的共生有效性与固氮作用
Plant Physiol. 1969 Dec;44(12):1666-8. doi: 10.1104/pp.44.12.1666.
3
Hydrogen evolution: A major factor affecting the efficiency of nitrogen fixation in nodulated symbionts.析氢：影响根瘤共生体中固氮效率的一个主要因素。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1207-11. doi: 10.1073/pnas.73.4.1207.
4
Purification and some properties of a non-haem iron protein from the bacteroids of soya-bean (Glycine max Merr) nodules.来自大豆（Glycine max Merr）根瘤类菌体的一种非血红素铁蛋白的纯化及某些性质
Biochem J. 1970 Aug;118(5):773-81. doi: 10.1042/bj1180773.
5
Nitrogenaseless mutants of Azotobacter vinelandii.棕色固氮菌的无氮突变体。
Proc Natl Acad Sci U S A. 1970 Jan;65(1):74-80. doi: 10.1073/pnas.65.1.74.
6
Effect of nitrogenase components from mutant and wild-type strains of Azotobacter on the dilution effect of nitrogenase.固氮菌突变株和野生型菌株的固氮酶组分对固氮酶稀释效应的影响。
Biochem J. 1971 Apr;122(3):305-9. doi: 10.1042/bj1220305.
7
Kinetic studies of nitrogenase from soya-bean root-nodule bacteroids.大豆根瘤类菌体中固氮酶的动力学研究。
Biochem J. 1973 Jan;131(1):61-75. doi: 10.1042/bj1310061.
8
Dinitrogen (N 2 ) fixation (with a biochemical emphasis).二氮（N₂）固定（侧重于生物化学方面）。
Bacteriol Rev. 1972 Jun;36(2):231-60. doi: 10.1128/br.36.2.231-260.1972.
9
Invitro formation of assimilatory reduced nicotinamide adenine dinucleotide phosphate: nitrate reductase from a Neurospora mutant and a component of molybdenum-enzymes.体外形成同化型还原型烟酰胺腺嘌呤二核苷酸磷酸：来自脉孢菌突变体的硝酸还原酶及钼酶的一个组分。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3242-6. doi: 10.1073/pnas.68.12.3242.
10
The mechanism of ammonia assimilation in nitrogen fixing Bacteria.固氮细菌中氨同化的机制。
Arch Mikrobiol. 1971;79(2):164-75. doi: 10.1007/BF00424923.
本文引用的文献
1
An electron donor system for nitrogenase-dependent acetylene reduction by extracts of soybean nodules.一种用于大豆根瘤提取物通过固氮酶还原乙炔的电子供体系统。
Plant Physiol. 1968 Sep;43(9):1458-60. doi: 10.1104/pp.43.9.1458.
2
NITROGEN FIXATION: HYDROSULFITE AS ELECTRON DONOR WITH CELL-FREE PREPARATIONS OF AZOTOBACTER VINELANDII AND RHODOSPIRILLUM RUBRUM.固氮作用：以连二亚硫酸盐作为电子供体，利用维涅兰德固氮菌和深红红螺菌的无细胞制剂
Proc Natl Acad Sci U S A. 1965 Mar;53(3):532-9. doi: 10.1073/pnas.53.3.532.
3
Nitrogen fixation by the bacteroid fraction of breis of soybean root nodules.大豆根瘤匀浆中类菌体部分的固氮作用。
Biochim Biophys Acta. 1967 Aug 29;141(3):507-15. doi: 10.1016/0304-4165(67)90179-1.
4
Purification, metal composition and properties of molybdoferredoxin and azoferredoxin, two of the components of the nitrogen-fixing system of Clostridium pasteurianum.巴氏芽孢梭菌固氮系统的两个组分，即钼铁氧还蛋白和偶氮铁氧还蛋白的纯化、金属组成及性质
Biochim Biophys Acta. 1967 Aug 29;141(3):516-22. doi: 10.1016/0304-4165(67)90180-8.
5
Reduction of acetylene and nitrogen gas by breis and cell-free extracts of soybean root nodules.大豆根瘤的菌苔和无细胞提取物对乙炔和氮气的还原作用。
Plant Physiol. 1967 Mar;42(3):466-8. doi: 10.1104/pp.42.3.466.
6
Some properties of nitrogen-fixing breis prepared from soybean root nodules.从大豆根瘤制备的固氮菌剂的一些特性。
Biochim Biophys Acta. 1966 Dec 28;130(2):304-12. doi: 10.1016/0304-4165(66)90225-x.
7
Components of cell-free extracts of Clostridium pasteurianum required for ATP-dependent H2 evolution from dithionite and for N2 fixation.巴氏梭菌无细胞提取物中对于连二亚硫酸盐依赖ATP的氢气释放及固氮作用所必需的成分。
Biochim Biophys Acta. 1966 Sep 26;127(1):18-25. doi: 10.1016/0304-4165(66)90470-3.
8
Acetylene reduction by nitrogen-fixing preparations from Clostridium pasteurianum.巴氏梭菌固氮制剂对乙炔的还原作用
Biochim Biophys Acta. 1966 Oct 31;127(2):285-94. doi: 10.1016/0304-4165(66)90383-7.
9
Method for demonstrating cofactor requirements for nitrogen fixation.证明固氮作用对辅助因子需求的方法。
Biochim Biophys Acta. 1965 Jun 15;104(1):278-81. doi: 10.1016/0304-4165(65)90245-x.
10
The adenosine triphosphate requirement for nitrogen fixation in cell-free extracts of Clostridium pasteurianum.巴氏芽孢杆菌无细胞提取物中固氮所需的三磷酸腺苷
Biochim Biophys Acta. 1965 Jun 22;99(3):486-503. doi: 10.1016/s0926-6593(65)80202-8.
Zotero 插件