Klucas R V, Koch B, Russell S A, Evans H J
Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331.
Plant Physiol. 1968 Dec;43(12):1906-12. doi: 10.1104/pp.43.12.1906.
The nitrogenase system in cell-free extracts of soybean nodule bacteroids was fractionated into 2 components by use of protamine sulfate or polypropylene glycol precipitation followed by chromatography on DEAE-cellulose. Iron and molybdenum were concentrated in 1 fraction and iron in the other. Combination of fractions resulted in a striking stimulation in activity relative to the activity of individual fractions. The effect of different proportions of the 2 fractions on specific activities was studied. The ratios of the rates of reduction of acetylene and N(2) by extracts or fractions of different purities were relatively constant. Extracts or fractions retained most of their nitrogenase activities when stored in liquid N(2).
通过使用硫酸鱼精蛋白或聚丙二醇沉淀,随后在DEAE - 纤维素上进行色谱分离,将大豆根瘤类菌体无细胞提取物中的固氮酶系统分成两个组分。铁和钼集中在一个组分中,而另一个组分中只有铁。组分的组合相对于各个组分的活性导致活性显著增强。研究了两种组分不同比例对比活性的影响。不同纯度的提取物或组分对乙炔和N₂的还原速率之比相对恒定。提取物或组分保存在液氮中时,大部分固氮酶活性得以保留。