Laboratory of Plant-Water Relations, Department of Water Science and Engineering, University of California, Davis, California 95616.
Plant Physiol. 1968 Dec;43(12):1947-52. doi: 10.1104/pp.43.12.1947.
This paper reports a consistent and large opening response to light + CO(2)-free air in living stomata of isolated epidermal strips of Vicia faba. The response was compared to that of non-isolated stomata in leaf discs floating on water; stomatal apertures, guard cell solute potentials and starch contents were similar in the 2 situations. To obtain such stomatal behavior, it was necessary to float epidermal strips on dilute KCl solutions. This suggests that solute uptake is necessary for stomatal opening.The demonstration of normal stomatal behavior in isolated epidermal strips provides a very useful system in which to investigate the mechanism of stomatal opening. It was possible to show independent responses in stomatal aperture to light and to CO(2)-free air.
本文报道了在分离的蚕豆表皮条中,活体气孔对光+无 CO2 空气的持续且大幅度的响应。该响应与在水面上浮游的叶圆片中非分离气孔的响应进行了比较;在这两种情况下,气孔开度、保卫细胞溶质势和淀粉含量相似。为了获得这种气孔行为,有必要将表皮条漂浮在稀 KCl 溶液上。这表明溶质摄取对于气孔张开是必要的。在分离的表皮条中证明正常的气孔行为提供了一个非常有用的系统,可以用来研究气孔张开的机制。有可能在气孔开度上对光和无 CO2 空气的响应是独立的。