Departments of Biology and Anatomy, Case Western Reserve University, Cleveland, Ohio 44106.
Plant Physiol. 1969 Jul;44(7):946-54. doi: 10.1104/pp.44.7.946.
A chloroplast fraction isolated from Acetabularia mediterrania carries on photosynthesis at rates essentially equal to those of whole cells. Electron and phase contrast microscopy reveals that the chloroplasts are intact and well preserved. Preparations contain no identifiable peroxisomes, but some cytoplasmic and mitochondrial contamination is present. Photosynthesis and CO(2) production in light by chloroplast preparations are in many respects similar to that of bean leaves, although the measured rates are somewhat lower. Respiration and photosynthesis of chloroplast preparations and whole cells of Acetabularia is essentially similar except that cells have a strong dark-type respiration which continues in light and is CO(2) dependent, the substrate being mainly recent photosynthate. The data suggest that chloroplasts are the site of photorespiration.
从地中海扇藻中分离出来的叶绿体在光合作用中的速率与整个细胞基本相等。电子和相差显微镜显示，叶绿体完整且保存良好。制备物中没有可识别的过氧化物酶体，但存在一些细胞质和线粒体污染。叶绿体制剂在光照下的光合作用和 CO2 产生在许多方面与豆叶相似，尽管测量的速率略低。除了细胞具有强烈的暗型呼吸作用，这种呼吸作用在光照下持续进行并依赖于 CO2 作为主要的近期光合产物外，叶绿体制剂和地中海扇藻的整个细胞的呼吸作用和光合作用基本相似。这些数据表明，叶绿体是光呼吸的场所。
