光照和年龄对绿体大麦叶片几种卡尔文循环酶分布的影响。
Influence of age and illumination on distribution of several calvin cycle enzymes in greening barley leaves.
机构信息
Department of Agronomy, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1970 May;45(5):579-82. doi: 10.1104/pp.45.5.579.
Activities of phosphoriboisomerase, phosphoribulokinase, and ribulose 1,5-diphosphate carboxylase, protein content, and chlorophyll accumulation in dark-grown barley seedlings were measured before and after illumination. Enzymatic activities, levels of soluble protein, and accumulation (upon illumination) of chlorophyll in leaves declined from tips toward the base. In response to increasing time of illumination, chlorophyll accumulation and activities of phosphoribulokinase and ribulose 1,5-diphosphate carboxylase (enzymes located in chloroplasts) increased most in tip portions whereas activity of phosphoriboisomerase and levels of soluble protein (constituents not confined to chloroplasts) increased similarly in all sections of the leaf. Maximum activity of phosphoribulokinase and maximum accumulation of chlorophyll shifted toward median portions of the leaf blade with increased age of seedling before illumination. Maximum activity of ribulose 1,5-diphosphate carboxylase and maximum level of soluble protein occurred in all leaf sections when the seedlings were 7 days of age before illumination.
在光照之前和之后，测量了黑暗中生长的大麦幼苗中磷酸核糖异构酶、磷酸核糖激酶和核酮糖 1,5-二磷酸羧化酶的活性、蛋白质含量和叶绿素积累。酶活性、可溶性蛋白水平以及叶片中叶绿素的积累（在光照下）从叶尖向叶基下降。随着光照时间的增加，叶绿素积累以及磷酸核糖激酶和核酮糖 1,5-二磷酸羧化酶（位于叶绿体中的酶）的活性在叶尖部分增加最多，而磷酸核糖异构酶的活性和可溶性蛋白的水平（不限于叶绿体的成分）在叶片的所有部分增加相似。在光照之前，随着幼苗年龄的增加，磷酸核糖激酶的最大活性和叶绿素的最大积累向叶片中部转移。在光照之前，当幼苗为 7 天时，所有叶片部分均出现了核酮糖 1,5-二磷酸羧化酶的最大活性和可溶性蛋白的最高水平。
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