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关于铜对小球藻损伤机制的观察。

Observations on the mechanism of copper damage in chlorella.

机构信息

Department of Life Sciences, University of California, Riverside, California 92502.

出版信息

Plant Physiol. 1970 Aug;46(2):183-5. doi: 10.1104/pp.46.2.183.

Abstract

Addition of excess copper to nongrowing cells of a normal, green Chlorella caused a reduction in total pigments and a blue shift of chlorophyll absorption, concurrent with the inhibition of photosynthesis. Chlorophylless yellow and white mutant strains of the same alga showed a rise in nonspecific absorption (i.e., change in light scatter) within 5 to 10 minutes after the addition of CuSO(4); concomitantly a lowering of packed cell volume and a rise in respiration occurred. Glutathione prevented all copper-induced changes, whereas MnCl(2) protected only partially. Selective inhibition of some responses to copper was observed when O(2) was absent or an antioxidant present.

摘要

向正常生长的绿藻非生长细胞中添加过量的铜会导致总色素减少,叶绿素吸收蓝移,同时光合作用受到抑制。同一藻类的叶绿素缺失的黄色和白色突变株在添加 CuSO(4) 后 5 至 10 分钟内表现出非特异性吸收(即光散射变化)增加;同时,细胞体积减小,呼吸作用增强。谷胱甘肽可防止所有由铜引起的变化,而 MnCl(2) 仅部分保护。当没有氧气或存在抗氧化剂时,观察到对铜的一些反应的选择性抑制。

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