The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel.
Plant Physiol. 1970 Aug;46(2):334-6. doi: 10.1104/pp.46.2.334.
No cytokinin activity was found in methanolic mesocarp extracts after purification with petroleum ether and ethyl acetate. Cytokinin activity did appear, however, in aqueous fraction after acid hydrolysis or passage through Dowex 50 (H(+)) ion exchange columns. The level of this activity, the fruit growth rate, and the cell division rate were found to be positively correlated (with each other).An inhibitor to cytokinin activity in soybean callus bioassay was found in the petroleum ether fraction; this inhibitor is not abscisic acid. The activity of the inhibitor could be considerably reduced by raising the level of kinetin in the nutrient medium. The intensity of inhibition was found to be positively correlated with reduction in the rate of mesocarp growth.The reduction in fruit growth rate may be considered to be due to the diminution of bound cytokinin activity and (or) increased activity of an endogenous inhibitor.
经石油醚和乙酸乙酯纯化后,甲醇法中层提取物中未发现细胞分裂素活性。然而,经酸水解或通过 Dowex 50(H(+))离子交换柱后,水相部分出现细胞分裂素活性。该活性水平、果实生长率和细胞分裂率呈正相关(彼此相关)。在大豆愈伤组织生物测定中发现,石油醚部分存在细胞分裂素活性抑制剂;该抑制剂不是脱落酸。通过在营养培养基中提高激动素水平,可大大降低抑制剂的活性。抑制强度与中层生长速率降低呈正相关。果实生长率降低可能是由于结合细胞分裂素活性降低和(或)内源性抑制剂活性增加所致。