Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Plant Physiol. 1973 Jul;52(1):68-71. doi: 10.1104/pp.52.1.68.
The levels of the activities of argininosuccinate synthetase and argininosuccinate lyase were measured in soybean (glycine max L. var. Mandarin) cell suspension cultures grown in the presence or absence of exogenous arginine. In some experiments, actinomycin D or cycloheximide were also added to the cultures, at critical stages of their growth. The results obtained led to the conclusion that activity of argininosuccinate synthetase is subject to significant inhibition by levels of arginine similar to those found to occur within the cells. Argininosuccinate lyase activity appeared to be enhanced, when arginine levels were increased above those occurring physiologically. Both enzymes appeared to be subject to inactivation, possibly via proteolysis.
在含有或不含有外源精氨酸的条件下,对大豆(Glycine max L. var. Mandarin)细胞悬浮培养物中的精氨酸琥珀酸合成酶和精氨酸琥珀酸裂解酶的活性水平进行了测量。在一些实验中,在细胞生长的关键阶段,还向培养物中添加了放线菌素 D 或环己酰亚胺。所得结果得出结论,精氨酸琥珀酸合成酶的活性受到与细胞内发生的水平相似的精氨酸水平的显著抑制。当精氨酸水平升高到生理水平以上时,精氨酸琥珀酸裂解酶的活性似乎增强。这两种酶似乎都容易失活,可能是通过蛋白水解作用。