Day D A, Wiskich J T
Department of Botany, University of Adelaide, Adelaide, 5001, Australia.
Plant Physiol. 1974 Jan;53(1):104-9. doi: 10.1104/pp.53.1.104.
Exogenous NADH oxidation by cauliflower (Brassica oleracea L.) bud mitochondria was sensitive to antimycin A and gave ADP/O ratios of 1.4 to 1.9. In intact mitochondria, NADH-cytochrome c reductase activity was only slightly inhibited by antimycin A. The antimycin-insensitive activity was associated with the outer membrane. Malate oxidation was sensitive to both rotenone and antimycin A and gave ADP/O values of 2.4 to 2.9. However in the presence of added NAD(+), malate oxidation displayed similar properties to exogenous NADH oxidation. In both the presence and absence of added NAD(+), malate oxidation was dependent on inorganic phosphate and inhibited by 2-n-butyl malonate.
花椰菜（Brassica oleracea L.）芽线粒体对外源NADH的氧化作用对抗霉素A敏感，ADP/O比值为1.4至1.9。在完整的线粒体中，NADH-细胞色素c还原酶活性仅受到霉素A的轻微抑制。对抗霉素不敏感的活性与外膜有关。苹果酸氧化对鱼藤酮和抗霉素A均敏感，ADP/O值为2.4至2.9。然而，在添加NAD(+)的情况下，苹果酸氧化表现出与外源NADH氧化相似的特性。无论是否添加NAD(+)，苹果酸氧化均依赖于无机磷酸盐，并受到2-正丁基丙二酸的抑制。
