Department of Vegetable Crops, University of California, Davis, California 95616.
Plant Physiol. 1974 Dec;54(6):809-12. doi: 10.1104/pp.54.6.809.
The major metabolite produced during incubation of [(3)H]gibberellin A(1) ([(3)H]GA(1)) with barley aleurone layers is an amphoteric, water-soluble compound tentatively called [(3)H]ampho GA(1). Formation of [(3)H]ampho GA(1) in barley aleurones begins after a period of 2.5 hours. As judged by degradation studies as well as Sephadex column chromatography, GA(1) appears to be linked to a peptide; positions C-3 and C-7 were ruled out as conjugation sites.
在大麦糊粉层中孵育 [(3)H]赤霉素 A1([(3)H]GA(1))时产生的主要代谢产物是一种两性、水溶性化合物,暂定名为 [(3)H]两性 GA(1)。在大麦糊粉层中,[(3)H]两性 GA(1)的形成始于 2.5 小时后。根据降解研究以及 Sephadex 柱层析判断,GA(1)似乎与肽相连;排除了 C-3 和 C-7 位置作为结合位点。