Hogsett W E, Quatrano R S
Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331.
Plant Physiol. 1975 Jan;55(1):25-9. doi: 10.1104/pp.55.1.25.
Beginning 10 hours after fertilization, zygotes of Fucus distichus L. Powell incorporate (35)S into polysaccharides as a sulfate ester of fucose. These sulfated polysaccharides are sequestered in only the rhizoid cell of the two-celled embryo and can serve as a marker of cellular differentiation. Zygotes were pulsed at different times after fertilization with Na(2) (35)SO(4) to identify and isolate the fucans localized within the region of cytoplasm destined to become the rhizoid cell. Low molecular weight pools of (35)S were saturated within 60 minutes, with the greatest incorporation into ethanol-soluble and insoluble fractions occurring with 0.1 mm Na(2)SO(4) in the artificial sea water medium. At the time of rhizoid formation, four fucose-containing polysaccharide fractions incorporated (35)S. When each fraction was subjected to diethylaminoethyl chromatography, two components were eluted with KCl that contained over 84% of the fucose and 93% of the (35)S of the particular fraction. Highvoltage paper electrophoresis of each fraction also resulted in the separation of these two major components. Both components from each of the four fractions behaved identically when separated by diethylaminoethyl chromatography and paper electrophoresis. By comparing the incorporation of (35)S into the polysaccharide fractions at 4 and 16 hours after fertilization, the fucan-sulfate components that are localized in the cytoplasm at the time of rhizoid formation were isolated. Although sulfated polysaccharides in brown algae are reported to be very heterogeneous in terms of their sugar composition and complexes with other heteropolymers, we propose that there are two major components that are sulfated during early embryogenesis in Fucus. The location of these two sulfated polysaccharides in different chemical fractions may reflect their subcellular localization (e.g., cytoplasmic vesicles or cell walls), or their association with other heteropolymers.
在受精后10小时开始，鹿角菜（Fucus distichus L. Powell）的合子将³⁵S掺入作为岩藻糖硫酸酯的多糖中。这些硫酸化多糖仅在二细胞胚的假根细胞中积累，可作为细胞分化的标志物。在受精后的不同时间用Na₂³⁵SO₄对合子进行脉冲处理，以鉴定和分离定位于注定成为假根细胞的细胞质区域内的岩藻聚糖。低分子量的³⁵S池在60分钟内饱和，在人工海水培养基中加入0.1 mM Na₂SO₄时，乙醇可溶性和不溶性部分的掺入量最大。在假根形成时，四个含岩藻糖的多糖部分掺入了³⁵S。当每个部分进行二乙氨基乙基色谱分析时，用KCl洗脱的两个组分含有特定部分中超过84%的岩藻糖和93%的³⁵S。每个部分的高压纸电泳也导致了这两个主要组分的分离。通过二乙氨基乙基色谱分析和纸电泳分离时，四个部分中每个部分的两个组分表现相同。通过比较受精后4小时和16小时³⁵S掺入多糖部分的情况，分离出了在假根形成时定位于细胞质中的岩藻糖硫酸酯组分。尽管据报道褐藻中的硫酸化多糖在糖组成和与其他杂聚物的复合物方面非常不均一，但我们提出在鹿角菜早期胚胎发生过程中有两个主要组分被硫酸化。这两种硫酸化多糖在不同化学部分中的位置可能反映了它们的亚细胞定位（例如，细胞质囊泡或细胞壁），或者它们与其他杂聚物的关联。
