Department of Biology, Technicon-Israel Institute of Technology, Haifa, Israel.
Plant Physiol. 1975 Feb;55(2):282-7. doi: 10.1104/pp.55.2.282.
Chloroplasts were treated with 2 m sodium bromide. The resulting particles lost their ATPase activity and chloroplast coupling factor 1 subunits were detected in the supernatant by means of gel electrophoresis and specific antibodies. The chloroplast coupling factor 1 depleted particles show high rates of Hill reaction with pH optimum shifted toward lower pH. The sodium bromide treatment also abolished the light-induced proton uptake. In the presence of N-methylphenazonium methosulfate light-induced proton release, insensitive to uncouplers, was observed. Addition of dicyclohexylcarbodiimide reversed the light-induced pH changes to the normal proton uptake and increased the pH optimum of the Hill reaction.
叶绿体用 2m 溴化钠处理。通过凝胶电泳和特异性抗体，在上清液中检测到失去 ATP 酶活性的叶绿体偶联因子 1 亚基。叶绿体偶联因子 1 耗尽的颗粒在 pH 值较低时表现出较高的希尔反应速率。溴化钠处理还消除了光诱导的质子吸收。在 N-甲基吩嗪甲硫酸盐存在下，观察到光诱导的质子释放，对解偶联剂不敏感。二环己基碳二亚胺的添加使光诱导的 pH 变化恢复为正常的质子吸收，并增加了希尔反应的 pH 值最优值。
