Department of Biology, University of California, San Diego, La Jolla, California 92037.
Plant Physiol. 1975 Feb;55(2):427-9. doi: 10.1104/pp.55.2.427.
Irradiation of a soluble extract from broken cells of Dictyostelium discoideum causes the photoreduction of a b-type cytochrome. The cytochrome b can be separated from cytochrome c, which is also present in the extract, by column chromatography on Brushite, but the cytochrome b is no longer sensitive to light after separation on the column. Low temperature spectroscopy shows that reduced form of the photoreducible cytochrome b has a Soret band at 423 nm and a split alpha band with maxima at 558 and 551 nm similar to the b-type cytochrome in complex II of beef heart mitochondria.
辐照离散型细胞的可溶性提取物会引起细胞色素 b 的光还原。细胞色素 b 可以通过 Brushite 柱层析从提取物中的细胞色素 c 中分离出来,但分离后细胞色素 b 对光不再敏感。低温光谱表明,可光还原的细胞色素 b 的还原形式在 423nm 处具有 Soret 带,在 558nm 和 551nm 处具有分裂的α带,类似于牛心线粒体复合物 II 中的 b 型细胞色素。