Pomeroy M K
Chemistry and Biology Research Institute, Research Branch, Agriculture Canada, Ottawa, Ontario, K1A OC6, Canada.
Plant Physiol. 1976 Apr;57(4):469-73. doi: 10.1104/pp.57.4.469.
A comparison of mitochondria isolated from 2 and 24 C grown winter wheat (Triticum aestivum L.) and winter rye (Secale cereale L.) seedlings revealed no correlation between changes in swelling and contraction characteristics and extent of cold hardiness. The swelling response changed markedly due to growth at low temperature, but the change was similar for the four cultivars examined. The swelling response was also observed to change rapidly during aging of isolated mitochondria, either at 2 or 24 C. Spontaneously swollen mitochondria, isolated from 24 C grown seedlings, contracted abruptly upon addition of certain oxidizable substrates, but this response was lost when seedlings were transferred from 24 to 2 C. Studies on the effect of various substrates and respiratory inhibitors on the swelling and contraction responses indicate that inhibitors which reduce or stop electron flow through the electron transport chain also inhibit substrate induced mitochondrial contraction.
对从2℃和24℃生长的冬小麦（Triticum aestivum L.）和冬黑麦（Secale cereale L.）幼苗中分离出的线粒体进行比较，结果显示肿胀和收缩特性的变化与抗寒程度之间没有相关性。由于在低温下生长，肿胀反应发生了显著变化，但在所检测的四个品种中变化相似。在2℃或24℃下，分离出的线粒体在老化过程中，肿胀反应也被观察到迅速变化。从24℃生长的幼苗中分离出的自发肿胀的线粒体，在添加某些可氧化底物后会突然收缩，但当幼苗从24℃转移到2℃时，这种反应就会消失。对各种底物和呼吸抑制剂对肿胀和收缩反应的影响的研究表明，减少或阻止电子通过电子传递链流动的抑制剂也会抑制底物诱导的线粒体收缩。
