玉米平脐蠕孢菌 Race T 毒素化学测定方法的评价。

Evaluation of a Chemical Method for Assay of Helminthosporium maydis Race T Toxin.

机构信息

Departments of Plant Pathology and Plant Breeding, Cornell University, Ithaca, New York 14853.

出版信息

Plant Physiol. 1977 May;59(5):792-4. doi: 10.1104/pp.59.5.792.

DOI:10.1104/pp.59.5.792

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC543298/

Abstract

A chemical test reported by Karr et al. (Plant Physiol. 55:727) to assay for host-specific toxin produced by Helminthosporium maydis race T was evaluated. Preparations from culture filtrates of both race T isolates, containing host-specific toxin, and similar preparations from race O isolates, containing no detectable host-specific toxin, gave positive reactions in the chemical assay. Also, preparations containing active or inactive toxin gave equal responses in this test. The procedure does not provide a reliable method for assaying H. maydis race T toxin.

摘要

Karr 等人报告的一种化学检测方法（植物生理学 55:727）用于检测玉米旋孢腔菌 T 小种产生的宿主特异性毒素，对此我们进行了评估。含有宿主特异性毒素的 T 小种分离株的培养滤液以及含有不可检测到的宿主特异性毒素的 O 小种分离株的类似培养滤液在化学检测中均呈阳性反应。此外，含有活性或非活性毒素的制剂在该试验中给出了相同的反应。该程序不能为玉米旋孢腔菌 T 小种毒素提供可靠的检测方法。

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本文引用的文献

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Inhibition of Dark CO(2) Fixation and Photosynthesis in Leaf Discs of Corn Susceptible to the Host-specific Toxin Produced by Helminthosporium maydis, Race T.对玉米叶圆片中暗CO₂固定和光合作用的抑制，该玉米对玉米长蠕孢菌T小种产生的寄主专化毒素敏感

Plant Physiol. 1975 Jul;56(1):1-7. doi: 10.1104/pp.56.1.1.

2

The Toxins of Helminthosporium maydis (Race T) : A Colorimetric Determination of the Toxins, Their Appearance in Culture and in Infected Plants.玉米旋孢腔菌（T 型）毒素：毒素的比色测定及其在培养物和感病植物中的出现。

Plant Physiol. 1975 Apr;55(4):727-30. doi: 10.1104/pp.55.4.727.

3

Isolation and Partial Characterization of Four Host-specific Toxins of Helminthosporium maydis (Race T).玉米小斑病菌（T小种）四种寄主专化性毒素的分离与部分特性鉴定

Plant Physiol. 1974 Feb;53(2):250-7. doi: 10.1104/pp.53.2.250.

4

The cholesterol content of the outer and inner membranes of guinea-pig liver mitochondria.豚鼠肝脏线粒体内膜和外膜的胆固醇含量。

Biochim Biophys Acta. 1967 May 2;135(2):362-4. doi: 10.1016/0005-2736(67)90132-0.

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