Charles F. Kettering Research Laboratory, 150 East South College Street, Yellow Springs, Ohio 45387.
Plant Physiol. 1978 May;61(5):753-6. doi: 10.1104/pp.61.5.753.
Rhizobium japonicum cells were grown in liquid suspension cultures and separated from soybean plant cells by two to three bacterial membrane filters. Under these conditions, the plant cells elaborated materials into the medium which aided in the expression of a major rhizobial phenotype, namely, nitrogenase activity (acetylene reduction). The evolution of H(2) was also measured and this activity relative to acetylene reduction, was influenced by: (a) O(2); (b) the quantity of conditioned plant medium; and (c) ammonia. It is concluded that plant substances are of major importance in the H(2) evolution and nitrogenase activities of free-living rhizobia in suspension cultures.
根瘤菌细胞在液体悬浮培养液中生长，并用两到三层细菌膜过滤器与大豆植物细胞分离。在这些条件下，植物细胞将物质分泌到培养基中，有助于表达主要的根瘤菌表型，即固氮酶活性（乙炔还原）。还测量了 H₂ 的演化，并且该活性相对于乙炔还原，受以下因素影响：(a) O₂；(b) 条件化植物培养基的量；和 (c) 氨。结论是，在悬浮培养中的自由生活根瘤菌的 H₂ 演化和固氮酶活性中，植物物质具有重要意义。
