Department of Biology, Bradley University, Peoria, Illinois 61625.
Plant Physiol. 1978 Jun;61(6):1031-3. doi: 10.1104/pp.61.6.1031.
The infectivity of Agrobacterium tumefaciens strain B6 was inhibited about 50% when these bacteria were inoculated on potato discs with equal viable cell counts of a weakly virulent strain of A. tumefaciens (B-48) or autoclaved strains of B6 or B-48. Inhibition by B-48 or autoclaved B6 could still be obtained when these cells were added up to a maximum of 10 minutes after the addition of viable B6. Maximum inhibition occurred when these cells were added 10 minutes prior to the addition of B6. There was no inhibition observed when equal cell counts of B6 were added along with a Gram-positive bacterium or yeast cell, while inhibition was observed when these B6 cells were added simultaneously with other Gram-negative cells. These results suggest that a physical, specific bacterial attachment that occurs within 10 minutes is necessary for tumor formation on potato discs.
当将具有同等活细胞数的弱毒力根癌农杆菌（B-48）菌株或巴氏消毒的 B6 或 B-48 菌株接种到马铃薯圆盘上时，根癌农杆菌 B6 菌株的感染力抑制了约 50%。当在添加具有活力的 B6 后最多 10 分钟内添加 B-48 或巴氏消毒的 B6 时，仍然可以获得 B-48 或巴氏消毒的 B6 的抑制作用。当这些细胞在添加 B6 之前 10 分钟添加时，抑制作用最大。当与革兰氏阳性细菌或酵母细胞一起添加等量的 B6 细胞时，没有观察到抑制作用，而当同时添加这些 B6 细胞与其他革兰氏阴性细胞时，观察到了抑制作用。这些结果表明，在 10 分钟内发生的物理的、特定的细菌附着是在马铃薯圆盘上形成肿瘤所必需的。
