Department of Plant Sciences, University of California, Riverside, California 92521.
Plant Physiol. 1979 Apr;63(4):677-9. doi: 10.1104/pp.63.4.677.
Plasma membranes from abscission zones of kidney bean seedlings were isolated on sucrose density gradients using cellulase and KCl-stimulated ATPase as marker enzymes. Following treatment of the seedling explants with 50 microliters per liter ethylene, an increase in the buoyant density of the plasma membrane fraction from 1.165 to 1.175 grams per cubic centimeter was observed. Such a change is consistent with the loss of lipids from the senescent cells of the abscission zone.
用纤维素酶和 KCl 刺激的 ATP 酶作为标记酶,从肾豆幼苗离层区分离质膜。用每升 50 微升乙烯处理幼苗外植体后,观察到质膜部分的浮力密度从 1.165 克/立方厘米增加到 1.175 克/立方厘米。这种变化与离层区衰老细胞中脂质的丧失一致。