Laboratory of Protoplasmatology, Department of Horticultural Science and Landscape Architecture, University of Minnesota, Saint Paul, Minnesota 55108.
Plant Physiol. 1979 Jul;64(1):131-8. doi: 10.1104/pp.64.1.131.
Effects of octylguanidine (OG) were studied on the permeability of cells of the adaxial epidermis of Allium cepa bulb scales to water and methyl urea and on the protoplast surface. Interference of OG with the Ca(2+) and Al(3+) action on the cell surface was also investigated.Permeability of the cell membrane for water and methyl urea increased nearly three times in presence of OG. The effect of OG on cell permeability depended on its direct contact with the protoplast surface.The effect of OG on the interaction between the protoplast surface and the cell wall (wall attachment) was marked and rapid; OG (225 micromolar) decreased the time for protoplast detachment in hypertonic solutions from 420 to 120 seconds. The plasmolyzed protoplasts were immediately rounded off while the controls without OG remained heavily concave.A considerable increase in protoplast detachment time and a decrease in rounding percentage were found when cells were plasmolyzed after pretreatment with AlCl(3) (0.05 molar for 2 minutes). This effect was partially reversed by KCl which was further enhanced by addition of OG.Penetration of OG into the mesoplasm was manifested only after 10 to 15 minutes. Vacuolization and swelling of the protoplasm, fragmentation of the protoplast, and aggregation of the spherosomes, however, were observed only 30 minutes after transfer. No evidence for penetration of OG into the vacuole was found.The results support earlier suggestions that OG acts primarily on the protoplast surface by interacting with membrane proteins as well as with phospholipids. In several aspects, OG acts on the cell surface similarly to a surfactant.
辛胍（OG）对洋葱鳞片表皮细胞的水和甲基尿素通透性以及原生质体表面的影响。还研究了 OG 对 Ca（2+）和 Al（3+）对细胞表面作用的干扰。在 OG 存在的情况下，细胞膜对水和甲基尿素的通透性增加了近三倍。OG 对细胞通透性的影响取决于其与原生质体表面的直接接触。OG 对原生质体表面与细胞壁相互作用（壁附着）的影响明显且迅速；OG（225 微摩尔）将原生质体在高渗溶液中分离的时间从 420 秒缩短至 120 秒。质壁分离的原生质体立即变圆，而没有 OG 的对照物仍严重凹陷。当细胞在用 AlCl3（0.05 摩尔，2 分钟）预处理后质壁分离时，发现原生质体分离时间显著延长，圆化率降低。这一效应部分被 KCl 逆转，OG 的加入进一步增强了这一效应。OG 渗透到胞质中仅在 10 至 15 分钟后才表现出来。然而，在转移后 30 分钟仅观察到质体的液泡化和肿胀、原生质体的碎裂以及球体的聚集。没有发现 OG 渗透到液泡中的证据。这些结果支持了早先的建议，即 OG 通过与膜蛋白以及与磷脂相互作用，主要作用于原生质体表面。在几个方面，OG 对细胞表面的作用类似于表面活性剂。
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