Department of Botany, Faculty of Science, University of Tokyo, Tokyo 113, Japan.
Plant Physiol. 1979 Jul;64(1):162-4. doi: 10.1104/pp.64.1.162.
Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were composed of 3 to 10 cells, to a medium containing zeatin (0.1 micromolar) but no auxin. The frequency of embryo formation reached more than 90%, and synchrony of the embryogenetic process was observed at least in the early stages of the process. The system established in the present work provides a useful system for biochemical research into the mechanisms of somatic embryogenesis.
体细胞胚胎发生的同步化是通过筛除初始异质细胞群体、在菲可溶液中进行密度梯度离心以及随后在低速（50g）短时间（5 秒）内进行多次离心来实现的，然后将获得的细胞团转移到含有玉米素（0.1 微摩尔）但不含生长素的培养基中。胚胎形成的频率达到 90%以上，并且在胚胎发生过程的早期至少观察到了胚胎发生过程的同步性。本工作中建立的体系为体细胞胚胎发生机制的生化研究提供了一个有用的体系。
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