Division of Laboratories and Research, New York State Department of Health, Albany, New York 12201.
Plant Physiol. 1979 Oct;64(4):525-7. doi: 10.1104/pp.64.4.525.
C-Phycocyanin was purified from an alga isolated in the Antarctic in 1968 and grown since that time in our laboratory at 4 C. Certain of its spectroscopic and immunochemical properties were studied, along with detailed examinations of its chemical composition and quaternary structure. The hexamer-trimer equilibrium in the protein solution was studied as a function of pH, ionic strength, temperature, and susceptibility to certain small molecules. A major emphasis of the work was placed on a comparison of its protein-protein interactions with those of C-phycocyanins from both mesophilic and thermophilic algae.
C-藻蓝蛋白于 1968 年从南极分离的藻类中纯化,并自此在我们位于 4°C 的实验室中进行培养。我们对其光谱和免疫化学性质进行了研究,并详细检查了其化学组成和四级结构。研究了蛋白质溶液中六聚体-三聚体平衡与 pH 值、离子强度、温度以及对某些小分子的敏感性的关系。这项工作的重点主要放在比较其蛋白质-蛋白质相互作用与来自嗜温和嗜热藻类的 C-藻蓝蛋白的相互作用。