Akamba L M, Anderson L E
Department of Biological Sciences, University of Illinois at Chicago Circle, Chicago, Illinois 60680.
Plant Physiol. 1981 Feb;67(2):197-200. doi: 10.1104/pp.67.2.197.
Light activation of NADP-linked glyceraldehyde-3-P dehydrogenase (EC 1.2.1.13) and light inactivation of glucose-6-P dehydrogenase (EC 1.1.1.49) appear to be modulated within pea leaf chloroplasts by mediators which are reduced by photosynthetic electron flow from the photosystem I reaction center. Dichlorophenyl-1, 1-dimethylurea inhibition of this modulation can be completely reversed by ascorbate plus 2,6-dichlorophenolindophenol in broken chloroplasts, but not in intact chloroplasts. Intact chloroplasts are impermeable to 2,6-dichlorophenolindophenol at pH 7.5. Studies on the effect of light in reconstituted chloroplasts with photosystem I-enriched particles in the place of whole thylakoids revealed that photosystem I participates in the light modulation of NADP-linked glyceraldehyde-3-P dehydrogenase and of glucose-6-P dehydrogenase.
与NADP相关的甘油醛-3-磷酸脱氢酶(EC 1.2.1.13)的光激活以及葡萄糖-6-磷酸脱氢酶(EC 1.1.1.49)的光失活,似乎在豌豆叶片叶绿体中受到一些介质的调节,这些介质会被来自光系统I反应中心的光合电子流还原。在破碎的叶绿体中,二氯苯基-1,1-二甲基脲对这种调节的抑制作用可被抗坏血酸加2,6-二氯酚靛酚完全逆转,但在完整的叶绿体中则不能。在pH 7.5时,完整的叶绿体对2,6-二氯酚靛酚是不可渗透的。用富含光系统I的颗粒代替完整类囊体对重建叶绿体进行光照效应研究,结果表明光系统I参与了与NADP相关的甘油醛-3-磷酸脱氢酶和葡萄糖-6-磷酸脱氢酶的光调节。