Plant Biology Program, Department of Biology, Washington University, St. Louis, Missouri 63130.
Plant Physiol. 1984 Feb;74(2):247-51. doi: 10.1104/pp.74.2.247.
The salt-extractable hydroxyproline-rich cell wall glycoprotein from carrot (Daucus carota L.) roots is composed of 35% (w/w) protein, 3% (w/w) galactose, and 62% (w/w) arabinose. The arabinose is attached to hydroxyproline as tetra- and trisaccharides. The circular dichroism of the glycoprotein shows that it is completely in the polyproline II conformation. After deglycosylation of the glycoprotein, the polyproline II conformation of the peptide backbone was lost. This indicates that the carbohydrate reinforces the polyproline II conformation.
胡萝卜(Daucus carota L.)根中的盐可提取的富含羟脯氨酸的细胞壁糖蛋白由 35%(w/w)的蛋白质、3%(w/w)的半乳糖和 62%(w/w)的阿拉伯糖组成。阿拉伯糖作为四糖和三糖连接到羟脯氨酸上。糖蛋白的圆二色性表明它完全处于聚脯氨酸 II 构象中。糖蛋白糖基化后,肽链的聚脯氨酸 II 构象丧失。这表明碳水化合物增强了聚脯氨酸 II 构象。