Australian Institute of Marine Science, PMB No. 3, TMC, Townsville, Australia 4810.
Plant Physiol. 1984 Jun;75(2):508-10. doi: 10.1104/pp.75.2.508.
Studies of the interactions of the large and small subunits of ribulose bisphosphate carboxylase require a knowledge of the concentrations of the subunits present in various preparations and their ratio. Since existing sodium dodecyl sulfate-gel electrophoresis procedures proved quantitatively unreliable, a technique based on high performance-gel filtration was developed. The latter is most reliable, takes only about 30 minutes to perform, and detects a minimum of 0.25 micrograms of each subunit.
研究核酮糖二磷酸羧化酶的大亚基和小亚基的相互作用需要了解各种制剂中存在的亚基的浓度及其比例。由于现有的十二烷基硫酸钠-凝胶电泳程序在定量上不可靠,因此开发了一种基于高性能凝胶过滤的技术。后者是最可靠的,只需要大约 30 分钟即可完成,并且可以检测到每个亚基的最小量为 0.25 微克。