Carnegie Institution of Washington Department of Plant Biology, Stanford, California 94305.
Plant Physiol. 1988 Dec;88(4):1397-402. doi: 10.1104/pp.88.4.1397.
Nuclear and cytoplasmic mRNAs for several phytochrome-regulated genes were examined in Pisum seedlings in order to investigate possible light effects on mRNA partitioning between the nucleus and cytoplasm. Transcripts from each of five light-regulated genes exhibited different responses to a variety of light treatments, but for each transcript we observed a characteristic linear relationship between nuclear and cytoplasmic levels over a wide range of total transcript abundance. Different mRNAs are characterized by different nuclear-cytoplasmic ;partitioning coefficients', indicating that post-transcriptional events play a significant role in regulating the accumulation of these mRNAs during light induction.
为了研究光照对核质间 mRNA 分配的可能影响,我们检测了豌豆幼苗中几种受光敏素调控的基因的核和细胞质 mRNA。五种受光调控基因的转录本对各种光照处理表现出不同的反应,但对于每个转录本,我们在广泛的总转录本丰度范围内观察到核和细胞质水平之间的特征线性关系。不同的 mRNA 具有不同的核质 ;分配系数',这表明转录后事件在调节这些 mRNA 在光诱导过程中的积累中起重要作用。