Eastern Regional Research Center, 600 East Mermaid Lane, Philadelphia, Pennsylvania 19118.
Plant Physiol. 1989 Feb;89(2):501-5. doi: 10.1104/pp.89.2.501.
Calmodulins (CaM) were isolated and characterized from two well-known latex producing plants, Papaver somniferum and Euphorbia lathyris. The molecular weights of both were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 17,000 comparable to that of bovine brain CaM. Amino acid compositions also compared similarly with those of known CaMs, with regard to the presence of trimethyllysine and the ratio of phenylalanine to tyrosine. The Cornish-Bowden equation (SDeltan) revealed strong statistical correlations of P. somniferum and E. lathyris CaM with those of other plants and animals, although their amino acid compositions were not identical. Both plant CaM stimulated CaM dependent cAMP phosphodiesterase: for Papaver somniferum the K(a) was found to be 1.09 nanomolar and for Euphorbia lathyris, 2.01 nanomolar.
钙调蛋白（CaM）从两种著名的产乳胶植物罂粟和大戟中被分离和鉴定。两者的分子量均通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定为 17000，与牛脑 CaM 相当。氨基酸组成也与已知的 CaM 相似，都存在三甲基赖氨酸和苯丙氨酸与酪氨酸的比值。尽管 Cornish-Bowden 方程（SDeltan）显示罂粟和大戟的 CaM 与其他植物和动物的 CaM 具有很强的统计学相关性，但它们的氨基酸组成并不相同。两种植物 CaM 都能刺激 CaM 依赖性 cAMP 磷酸二酯酶：罂粟 CaM 的 K(a)为 1.09 纳摩尔，大戟 CaM 的 K(a)为 2.01 纳摩尔。
