Department of Plant Nutrition, Aula Dei Experimental Station (C.S.I.C.), Apdo. 202, 50080 Zaragoza, Spain.
Plant Physiol. 1989 Sep;91(1):190-2. doi: 10.1104/pp.91.1.190.
A new reversed phase-high performance liquid chromatography method has been developed to analyze the full complement of higher plant photosynthetic pigments (cis-neoxanthin, neoxanthin, violaxanthin, taraxanthin, anteraxanthin, lutein, zeaxanthin, cis-lutein, chlorophyll b, chlorophyll a, alpha- and beta-carotene). The separation is carried out on a C(18) column in about 10 minutes, using a single high-pressure pump and three different mobile phases in three isocratic steps. This method introduces a major improvement in higher plant photosynthetic pigment analysis, resolving in only 10 minutes all photosynthetic pigments while achieving good separation of lutein from its isomer zeaxanthin. Zeaxanthin is involved in the xanthophyll cycle, which recently has been proposed to play a significant role in the protection of the photosynthetic apparatus from photoinhibitory conditions (Demmig et al. [1987] Plant Physiol 84: 218-224).
一种新的反相高效液相色谱法已被开发出来,用于分析全植物光合作用色素(顺式新黄质、新黄质、玉米黄质、紫黄质、玉米黄质、叶黄素、玉米黄素、顺式叶黄素、叶绿素 b、叶绿素 a、α-和β-胡萝卜素)。该分离在 C(18)柱上进行,大约 10 分钟,使用单一高压泵和三个不同的流动相在三个等度步骤中进行。该方法在高等植物光合作用色素分析方面引入了重大改进,仅需 10 分钟即可解析所有光合作用色素,同时实现叶黄素与其异构体玉米黄素的良好分离。玉米黄素参与叶黄素循环,该循环最近被提出在保护光合作用装置免受光抑制条件方面发挥重要作用(Demmig 等人,[1987]植物生理学 84:218-224)。