Plant Gene Expression Center, U.S. Department of Agriculture-Agricultural Research Service/University of California at Berkeley, Albany, California 94710.
Plant Physiol. 1989 Sep;91(1):440-4. doi: 10.1104/pp.91.1.440.
Intact maize cells were bombarded with microprojectiles bearing plasmid DNA coding for selectable (neomycin phosphotransferase [NPT II]) and screenable (beta-glucuronidase [GUS]) marker genes. Kanamycin-resistant calli were selected from bombarded cells, and these calli carried copies of the NPT II and GUS genes as determined by Southern blot analysis. All such calli expressed GUS although the level of expression varied greatly between transformed cell lines. These results show that intact cells of important monocot species can be stably transformed by microprojectiles.
完整的玉米细胞被带有编码可选择（新霉素磷酸转移酶[NPT II]）和可筛选（β-葡糖苷酸酶[GUS]）标记基因的微弹粒轰击。从被轰击的细胞中选择卡那霉素抗性愈伤组织，这些愈伤组织携带 NPT II 和 GUS 基因的拷贝，如 Southern 印迹分析所示。所有这些愈伤组织都表达了 GUS，尽管转化细胞系之间的表达水平差异很大。这些结果表明，重要的单子叶植物的完整细胞可以通过微弹粒稳定转化。
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