U.S. Department of Agriculture/Agricultural Research Service, Wheat Genetics, Quality, Physiology and Disease Research, Washington State University, Pullman, Washington 99164-6420.
Plant Physiol. 1991 Mar;95(3):814-21. doi: 10.1104/pp.95.3.814.
Hydrated dormant cereal seeds do not germinate even when environmental conditions are favorable for germination. By using cDNA cloning and differential screening, we have identified mRNAs from five gene families that are abundant in the embryos of imbibed, but developmentally arrested wheat (Triticum aestivum L.) seeds. Gene transcript levels of these mRNAs are maintained and even increase in embryos of imbibed dormant seeds for as long as the seeds remain dormant. In contrast, transcript levels decline in nondormant seeds after imbibition and disappear as germination occurs. All the identified genes are ABA responsive. Based on these data we conclude that wheat seeds in the hydrated dormant state exhibit prolonged expression of ABA-responsive genes.
水合休眠的谷物种子即使在有利于发芽的环境条件下也不会发芽。通过使用 cDNA 克隆和差异筛选，我们从吸水但发育停滞的小麦（Triticum aestivum L.）种子的胚胎中鉴定出了五个基因家族的丰富 mRNA。在吸水休眠种子的胚胎中，这些 mRNA 的基因转录水平得以维持，甚至在种子保持休眠状态时还会增加。相比之下，在非休眠种子吸水后，转录水平下降，并且随着发芽的发生而消失。所有鉴定出的基因都对 ABA 有反应。基于这些数据，我们得出结论，处于水合休眠状态的小麦种子表现出 ABA 反应基因的延长表达。
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