Castillo Claudia G, Mendoza Soledad, Freed William J, Giordano Magda
Department of Cognitive and Behavioural Neurobiology, Instituto de Neurobiología, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro.
Behav Brain Res. 2006 Jul 15;171(1):109-15. doi: 10.1016/j.bbr.2006.03.025. Epub 2006 May 4.
Repeated systemic administration of low doses of kainic acid (KA) induces spontaneous convulsive seizures [Hellier JL, Patrylo PR, Buckmaster PS, Dudek FE. Recurrent spontaneous motor seizures after repeated low-dose systemic treatment with kainate: assessment of a rat model of temporal lobe epilepsy. Epilepsy Res 1998;31:73-84]. In this study, male Sprague-Dawley animals received intranigral transplants of a control cell line M213-2O, or a cell line transfected with human GAD67 cDNA (M213-2O CL4) [Conejero-Goldberg C, Tornatore C, Abi-Saab W, Monaco MC, Dillon-Carter O, Vawter M, et al. Transduction of human GAD67 cDNA into immortalized striatal cell lines using an Epstein-Barr virus-based plasmid vector increases GABA content. Exp Neurol 2000;161:453-61], or no transplant. Eight weeks after transplantation surgery, KA was administered (5 mg/kg/h) until animals reached stage V seizures as described by Racine [Racine RJ. Modification of seizure activity by electrical stimulation. II. Motor seizure. Electroencephalogr Clin Neurophysiol 1972;32:281-94]. The group transplanted with CL4 required a larger dose of KA and a longer latency to reach a stage V seizure. In addition, this group exhibited significantly fewer stage III and IV seizures. These results indicate that intranigral transplants of a GABA-producing cell line can decrease the number of kainic acid-induced seizures.
反复低剂量全身注射海藻酸（KA）可诱发自发性惊厥发作[赫利尔JL，帕特里洛PR，巴克马斯特PS，杜德克FE。反复低剂量全身注射海藻酸盐后反复出现的自发性运动性癫痫发作：颞叶癫痫大鼠模型的评估。癫痫研究1998；31：73 - 84]。在本研究中，雄性斯普拉格 - 道利动物接受黑质内移植对照细胞系M213 - 2O，或转染人GAD67 cDNA的细胞系（M213 - 2O CL4）[科内杰罗 - 戈德堡C，托纳托雷C，阿比 - 萨布W，莫纳科MC，狄龙 - 卡特O，沃特M等。使用基于爱泼斯坦 - 巴尔病毒的质粒载体将人GAD67 cDNA转导到永生化纹状体细胞系中可增加GABA含量。实验神经病学2000；161：453 - 61]，或不进行移植。移植手术后8周，按照拉辛[拉辛RJ。电刺激对癫痫活动的改变。II。运动性癫痫。脑电图临床神经生理学1972；32：281 - 94]所述给予KA（5mg/kg/h），直至动物达到V期癫痫发作。移植CL4的组达到V期癫痫发作需要更大剂量的KA和更长的潜伏期。此外，该组出现的III期和IV期癫痫发作明显较少。这些结果表明，产生GABA的细胞系黑质内移植可减少海藻酸诱导的癫痫发作次数。
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