病原体铜绿假单胞菌会对秀丽隐杆线虫对细菌的吸引反应产生负面影响。

The pathogen Pseudomonas aeruginosa negatively affects the attraction response of the nematode Caenorhabditis elegans to bacteria.

作者信息

Laws Thomas R, Atkins Helen S, Atkins Timothy P, Titball Richard W

机构信息

Defence Science and Technology Laboratory, Porton Down, Salisbury, Wiltshire SP4 OJQ, UK.

出版信息

Microb Pathog. 2006 Jun;40(6):293-7. doi: 10.1016/j.micpath.2006.03.001. Epub 2006 May 6.

DOI:10.1016/j.micpath.2006.03.001

PMID:16678995

Abstract

The nematode Caenorhabditis elegans has previously been used to identify virulence mechanisms of bacteria and to characterise host responses to infection. In this study, we have developed an assay to measure C. elegans attraction to bacterial food sources. C. elegans becomes less attracted to the bacterial pathogen Pseudomonas aeruginosa strain PA14 over time, but this response is not seen with P. aeruginosa strains PAK1 or PA01. P. aeruginosa strain PA14 cells that had been killed by UV light, or which had been exposed to chloramphenicol, did not mediate this effect. We therefore propose that C. elegans reacts to a factor produced by P. aeruginosa strain PA14.

摘要

线虫秀丽隐杆线虫此前已被用于鉴定细菌的致病机制，并表征宿主对感染的反应。在本研究中，我们开发了一种测定方法来测量秀丽隐杆线虫对细菌食物来源的吸引力。随着时间的推移，秀丽隐杆线虫对细菌病原体铜绿假单胞菌PA14菌株的吸引力会降低，但对铜绿假单胞菌PAK1或PA01菌株则未观察到这种反应。经紫外线杀死或暴露于氯霉素的铜绿假单胞菌PA14菌株细胞不会介导这种效应。因此，我们提出秀丽隐杆线虫会对铜绿假单胞菌PA14菌株产生的一种因子做出反应。