González Antonio J, Hernández Domingo, De Vera Antonia, Barrios Ysamar, Salido Eduardo, Torres Armando, Terrados Nicolás
Service of Sport Medicine, Ayuntamiento Puerto de la Cruz, Hospital Universitario de Canarias and Instituto Reina Sofía de Investigación, La Laguna, Tenerife, Spain.
Med Sci Sports Exerc. 2006 Apr;38(4):688-93. doi: 10.1249/01.mss.0000210187.62672.b3.
To determine the role of the ACE (I/D) gene polymorphism on erythropoietic response in endurance athletes after natural exposure to moderate altitude.
Erythropoietic activity was measured in 63 male endurance athletes following natural exposure to moderate altitude (2200 m) during 48 h. Erythropoietin (EPO) levels and hemoglobin (Hb) concentrations were measured at baseline and 12, 24, and 48 h after reaching the set altitude. Reticulocyte counts were determined at baseline and 48 h thereafter. Subjects were grouped into two groups (responders and nonresponders) based on significant increase in EPO levels (median: > 16.5 ng x m(-1)) after 24 h at altitude. ACE gene polymorphism was ascertained by polymerase chain reaction (DD, 31 (49%); ID, 24 (38%); II, 8 (13%)).
Overall, EPO levels significantly increased at 12 (70%; P = 0.0001) and 24 h (72%; P = 0.0001) above baseline concentration following exposure to 2200 m. Thereafter, EPO concentration decreased at 48 h, but a significant increase in Hb levels (4.6 +/- 4%; P = 0.0001) and reticulocyte count (50.5 +/- 79%; P = 0.0001) was observed at the end of the experiment, suggesting negative feedback. There were no significant differences in EPO and Hb concentration profiles between subjects with DD genotype and those with other genotypes (ID/II). Moreover, responders (N = 42; DD, 50%; ID/II, 50%) and nonresponders (N = 21; DD, 48%; ID/II, 52%) showed a similar erythropoietic profile during the experiment and the ACE gene polymorphism did not influence the time course of the erythropoietic response.
The ACE gene polymorphism does not influence erythropoietic activity in endurance athletes after short-term exposure to moderate altitude.
确定血管紧张素转换酶（ACE）（I/D）基因多态性对耐力运动员在自然暴露于中度海拔后红细胞生成反应的作用。
在63名男性耐力运动员自然暴露于中度海拔（2200米）48小时后测量红细胞生成活性。在到达设定海拔的基线、12小时、24小时和48小时时测量促红细胞生成素（EPO）水平和血红蛋白（Hb）浓度。在基线和此后48小时测定网织红细胞计数。根据海拔24小时后EPO水平显著升高（中位数：>16.5 ng·m⁻¹）将受试者分为两组（反应者和无反应者）。通过聚合酶链反应确定ACE基因多态性（DD型，31例（49%）；ID型，24例（38%）；II型，8例（13%））。
总体而言，暴露于2200米后，EPO水平在高于基线浓度的12小时（70%；P = 0.0001）和24小时（72%；P = 0.0001）时显著升高。此后，EPO浓度在48小时时下降，但在实验结束时观察到Hb水平显著升高（4.6±4%；P = 0.0001）和网织红细胞计数显著升高（50.5±79%；P = 0.0001），提示负反馈。DD基因型受试者与其他基因型（ID/II）受试者之间的EPO和Hb浓度曲线无显著差异。此外，反应者（N = 42；DD型，50%；ID/II型，50%）和无反应者（N = 21；DD型，48%；ID/II型，52%）在实验期间表现出相似的红细胞生成特征，且ACE基因多态性不影响红细胞生成反应的时间进程。
短期暴露于中度海拔后，ACE基因多态性不影响耐力运动员的红细胞生成活性。
