前列腺特异性抗原（PSA）激活固有免疫
Activation of innate immunity by prostate specific antigen (PSA).
作者信息
Kodak James A, Mann Dean L, Klyushnenkova Elena N, Alexander Richard B
机构信息
Division of Urology, University of Maryland School of Medicine and VA Maryland Health Care System, Baltimore, Maryland 21201, USA.
出版信息
Prostate. 2006 Nov 1;66(15):1592-9. doi: 10.1002/pros.20414.
BACKGROUND
Prostate specific antigen (PSA) is a serine protease secreted by the prostatic epithelium. The only known function of the protein is to cleave seminogelin. We wished to determine if PSA activated peripheral blood mononuclear cells (PBMC).
METHODS
PBMC and selected sub-populations were cultured with purified PSA. Secretion of IFNgamma was measured by cytokine capture flow cytometry and enzyme-linked immunosorbent assay.
RESULTS
We observed secretion of IFNgamma and a proliferative response in PBMC cultured with PSA. We found that NK cells were the source of the IFNgamma but NK cells were not directly stimulated by PSA. Rather, a soluble factor secreted primarily by CD14 monocytes in response to PSA stimulated NK cells to secrete IFNgamma.
DISCUSSION
PSA induces a pro-inflammatory response that results in the secretion of INFgamma by NK cells. The presence of large amounts of PSA could contribute to the common finding of inflammatory infiltrates in the prostate.
背景
前列腺特异性抗原（PSA）是一种由前列腺上皮分泌的丝氨酸蛋白酶。该蛋白唯一已知的功能是裂解精囊蛋白。我们希望确定PSA是否能激活外周血单个核细胞（PBMC）。
方法
将PBMC及选定的亚群与纯化的PSA一起培养。通过细胞因子捕获流式细胞术和酶联免疫吸附测定法测量γ干扰素的分泌。
结果
我们观察到在用PSA培养的PBMC中有γ干扰素的分泌和增殖反应。我们发现NK细胞是γ干扰素的来源，但NK细胞不是由PSA直接刺激的。相反，主要由CD14单核细胞响应PSA分泌的一种可溶性因子刺激NK细胞分泌γ干扰素。
讨论
PSA诱导一种促炎反应，导致NK细胞分泌INFγ。大量PSA的存在可能是前列腺中常见炎性浸润的原因。
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