采用固相微萃取结合气相色谱 - 质谱联用技术对啤酒挥发性成分进行分析的方法优化。
Method optimization by solid-phase microextraction in combination with gas chromatography with mass spectrometry for analysis of beer volatile fraction.
作者信息
Pinho Olivia, Ferreira Isabel M P L V O, Santos Lúcia H M L M
机构信息
REQUIMTE-Serviço de Bromatologia, Faculdade de Farmácia da Universidade do Porto, Rua Anibal Cunha 164, 4099-030 Porto, Portugal.
出版信息
J Chromatogr A. 2006 Jul 21;1121(2):145-53. doi: 10.1016/j.chroma.2006.04.013. Epub 2006 May 9.
A simple and sensitive method for the analysis of beer volatile compounds was optimised using headspace solid-phase microextraction (SPME) and gas chromatography with mass detection. Headspace SPME using a 75 microm Carboxen-polydimethylsiloxane (CAR-PDMS) fiber provided effective sample enrichment and enabled extraction of a wide variety of compounds. The reproducibility depended on the compounds, with a mean value of 1.4% for alcohols, 3.3% for ethers, 6.7% for aldehydes, 3.4% for acids, 1.7% for aromatic compounds, 2.4% for esters, 7.4% for hydrocarbons, 1.8% for alicyclic compounds, and 3.4% for heterocyclic compounds. The optimised methodology can be used to compare volatile profile from different types of beers and eventually to study the evolution of a particular beer during aging.
采用顶空固相微萃取（SPME）和气相色谱 - 质谱检测技术，优化了一种用于分析啤酒挥发性化合物的简单灵敏方法。使用75微米的碳分子筛 - 聚二甲基硅氧烷（CAR - PDMS）纤维进行顶空固相微萃取，可有效富集样品，并能萃取多种化合物。重现性因化合物而异，醇类的平均值为1.4%，醚类为3.3%，醛类为6.7%，酸类为3.4%，芳香族化合物为1.7%，酯类为2.4%，烃类为7.4%，脂环族化合物为1.8%，杂环化合物为3.4%。优化后的方法可用于比较不同类型啤酒的挥发性特征，并最终研究特定啤酒在陈酿过程中的变化。
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