Suzuki S O, Iwaki T, Kitamoto T, Mizoguchi M, Fukui M, Tateishi J
Departments of Neuropathology and Neurosurgery, Neurological Institute, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Clin Mol Pathol. 1996 Jun;49(3):M140-6. doi: 10.1136/mp.49.3.m140.
Aims/background-CD44 is a widely distributed cell surface molecule which has numerous isoforms generated by alternative splicing. The diverse functions related to the CD44 variants (CD44v) have been reported in various physiological and pathological conditions. The pattern of expression of CD44v among meningioma subtypes was investigated to ascertain whether CD44 variants play a role in a variety of biological processes, such as epithelial differentiation and extracranial metastasis.Methods-Twenty three meningiomas were studied immunohistochemically using novel antibodies directed against CD44 isoforms. Six of the 23 samples were analysed by reverse transcription polymerase chain reaction (RT-PCR), followed by Southern blotting with CD44v specific probes.Results-In meningothelial, fibrous and anaplastic meningiomas, a standard form of CD44 was detected by RT-PCR and was homogeneously expressed in tumour cells when studied immunohistochemically. CD44v was not detected in these subtypes. In secretory meningiomas, however, CD44v isoforms were strongly expressed in the cell clusters that produce secretory granules and also accumulated in the granules. The population of tumour cells immunopositive for CD44v was similar to that which stained with antibodies directed against carcinoembryonic antigen, epithelial membrane antigen and ezrin. On RT-PCR with Southern blotting, only the secretory type showed high level expression of CD44v.Conclusions-CD44v in meningiomas is expressed in relation to tumour cell differentiation towards the epithelial type.
目的/背景——CD44是一种广泛分布的细胞表面分子，通过可变剪接产生众多异构体。在各种生理和病理条件下，已报道了与CD44变体（CD44v）相关的多种功能。研究脑膜瘤亚型中CD44v的表达模式，以确定CD44变体是否在多种生物学过程中发挥作用，如上皮分化和颅外转移。
方法——使用针对CD44异构体的新型抗体，对23例脑膜瘤进行免疫组织化学研究。对23个样本中的6个进行逆转录聚合酶链反应（RT-PCR）分析，随后用CD44v特异性探针进行Southern印迹分析。
结果——在脑膜内皮型、纤维型和间变性脑膜瘤中，通过RT-PCR检测到一种标准形式的CD44，免疫组织化学研究显示其在肿瘤细胞中均匀表达。在这些亚型中未检测到CD44v。然而，在分泌型脑膜瘤中，CD44v异构体在产生分泌颗粒的细胞簇中强烈表达，并且也在颗粒中积累。对CD44v呈免疫阳性的肿瘤细胞群体与用针对癌胚抗原、上皮膜抗原和埃兹蛋白的抗体染色的群体相似。通过Southern印迹的RT-PCR分析，只有分泌型显示出CD44v的高水平表达。
结论——脑膜瘤中的CD44v表达与肿瘤细胞向上皮型分化有关。
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