Ginther O J, Gastal E L, Gastal M O, Beg M A
Eutheria Foundation, Cross Plains, WI 53528, United States.
Domest Anim Endocrinol. 2007 May;32(4):315-28. doi: 10.1016/j.domaniend.2006.04.006. Epub 2006 Apr 27.
The effect of a single injection of prostaglandin F2alpha (PGF) during mid-diestrus on systemic concentrations of progesterone, LH, FSH, estradiol, and cortisol and on blood flow to the corpus luteum was studied in 10 controls and 10 PGF-treated mares. Blood flow was assessed by estimating the percentage of corpus luteum with color-Doppler signals of blood flow during real-time scanning of the entire structure and by the diameter of the vascular pedicle near its attachment to the ovary. Treatment was done 8 days after ovulation and 0 h was immediately before the treatment. Examinations and collection of blood samples were done at 0 h, every 5 min until 1h, and then at 1.5, 2, 4, 8, 12, 24, 48, and 72 h. The concentrations of estradiol did not change, but progesterone, LH, FSH, and cortisol increased significantly within 5 min. Concentrations of LH and FSH in the PGF group remained elevated until a temporarily lower concentration at 8 or 4h, respectively, rebounded to 12h, and then slowly decreased. Cortisol remained elevated, until a decrease between 1 and 4h. Progesterone in the PGF group increased significantly until 10 min after 0 h and then decreased by 40 min to below the concentrations in controls. Within the PGF group, progesterone decreased significantly by 45 min to below the concentrations at 0 h. The values for each of the two indicators of blood flow did not differ significantly between the PGF and control groups until a decrease at 24h in the PGF group. Results did not support the hypothesis that the immediate transient post-PGF increase in progesterone was associated with an increase in luteal blood flow. Luteolysis, as indicated by decreasing progesterone, began well before the beginning of a decrease in luteal blood flow.
研究了在发情周期中期向10匹对照母马和10匹接受前列腺素F2α（PGF）治疗的母马单次注射PGF对孕酮、促黄体生成素（LH）、促卵泡生成素（FSH）、雌二醇和皮质醇的全身浓度以及黄体血流量的影响。通过在对整个黄体结构进行实时扫描时估计有血流彩色多普勒信号的黄体百分比以及靠近其与卵巢连接处的血管蒂直径来评估血流量。排卵后8天进行治疗，治疗前0小时为即刻。在0小时、直至1小时每5分钟、然后在1.5、2、4、8、12、24、48和72小时进行检查并采集血样。雌二醇浓度未发生变化，但孕酮、LH、FSH和皮质醇在5分钟内显著升高。PGF组中LH和FSH的浓度一直升高，直到分别在8小时或4小时暂时降低，在12小时反弹，然后缓慢下降。皮质醇一直升高，直到在1至4小时之间下降。PGF组中的孕酮在0小时后10分钟内显著升高，然后在40分钟时下降至低于对照组的浓度。在PGF组内，孕酮在45分钟时显著下降至低于0小时时的浓度。在PGF组24小时出现下降之前，PGF组和对照组之间血流量的两个指标的值均无显著差异。结果不支持PGF后孕酮立即短暂升高与黄体血流量增加相关的假设。如孕酮下降所示，黄体溶解在黄体血流量开始下降之前就已开始。
