Verminnen Kristel, Van Loock Marnix, Hafez Hafez Mohamed, Ducatelle Richard, Haesebrouck Freddy, Vanrompay Daisy
Laboratory of Immunology and Animal Biotechnology, Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure links 653, Ghent 9000, Belgium.
Vet Res. 2006 Jul-Aug;37(4):623-32. doi: 10.1051/vetres:2006023. Epub 2006 May 16.
Chlamydophila psittaci (formerly Chlamydia psittaci) is one of the major pathogens associated with turkey respiratory disease. Devastating outbreaks with high mortality rates, similar to those of 1950 to 1970 in the USA occasionally occur, but respiratory signs without or with low mortality mostly characterize outbreaks now a day. Accurate diagnostic methods should be made available. The present study examined the sensitivity and specificity of a recombinant ELISA (rMOMP ELISA) for detecting Cp. psittaci major outer membrane specific antibodies in turkey sera. Test results were compared to those of immunoblotting and of a competitive ELISA (Chlamydia-psittaci-AK-EIA, Röhm Pharma, Germany) and an indirect ELISA (LPS/LGP) detecting antibodies to the lipopolysaccharide/lipoglycoprotein complex. The rMOMP ELISA was most sensitive as determined on serial dilutions of positive control sera originating from experimentally infected SPF turkeys. The competitive ELISA gave false positives since three negative controls reacted positive. For conventional sera, the sensitivities of the competitive ELISA, immunoblotting and the indirect ELISA were found to be 99.4, 93.1 and 82.2%, respectively, as compared to the rMOMP ELISA (100%). The specificities of the rMOMP ELISA, immunoblotting and the indirect ELISA were found to be 100% while the specificity of the competitive ELISA was only 2.7%. The rMOMP ELISA was chosen to compare the prevalence of chlamydiosis in 2002 with the one from 1992. In 2002, 188 on 200 (94%) turkey sera reacted positive compared to 175 on 200 (87.5%) in 1992 and like 10 years ago all examined farms were seropositive at slaughter. Interestingly, Belgian as well as French farms were seropositive.
鹦鹉热衣原体（以前称为鹦鹉衣原体）是与火鸡呼吸道疾病相关的主要病原体之一。偶尔会发生死亡率很高的毁灭性疫情，类似于美国1950年至1970年期间的情况，但如今疫情大多以无死亡或低死亡率的呼吸道症状为特征。应提供准确的诊断方法。本研究检测了重组ELISA（rMOMP ELISA）检测火鸡血清中鹦鹉热衣原体主要外膜特异性抗体的敏感性和特异性。将检测结果与免疫印迹法、竞争性ELISA（衣原体-鹦鹉热-AK-EIA，德国罗姆制药公司）以及检测脂多糖/脂糖蛋白复合物抗体的间接ELISA（LPS/LGP）的结果进行比较。对源自实验感染的无特定病原体（SPF）火鸡的阳性对照血清进行系列稀释后测定，rMOMP ELISA最为敏感。竞争性ELISA出现了假阳性，因为三个阴性对照呈阳性反应。对于常规血清，与rMOMP ELISA（100%）相比，竞争性ELISA、免疫印迹法和间接ELISA的敏感性分别为99.4%、93.1%和82.2%。rMOMP ELISA、免疫印迹法和间接ELISA的特异性均为100%，而竞争性ELISA的特异性仅为2.7%。选择rMOMP ELISA来比较2002年与1992年衣原体病的流行情况。2002年，200份火鸡血清中有188份（94%）反应呈阳性，而1992年200份中有175份（87.5%），并且与10年前一样，所有检测的农场在屠宰时血清均呈阳性。有趣的是，比利时和法国的农场血清均呈阳性。
