Cella Nathalie, Contreras Alejandro, Latha Khatri, Rosen Jeffrey M, Zhang Ming
Baylor College of Medicine, Department of Molecular and Cellular Biology, ALKEK Bldg., Rm. N630, One Baylor Plaza, Houston, Texas 77030, USA.
FASEB J. 2006 Jul;20(9):1510-2. doi: 10.1096/fj.05-5500fje. Epub 2006 May 23.
Maspin is a tumor-suppressor serpin (serine protease inhibitor), which inhibits cell invasion and migration. Here, we analyzed maspin function in cell adhesion in nontransformed mammary epithelial cells and investigated the underlying mechanism involved in this process. We report that maspin acts in the early steps in the cell adhesion process. Addition of recombinant maspin rapidly increased MCF-10A cell adhesion to the endogenously deposited matrix, and conversely both an antimaspin antibody (Ab) and maspin knockdown by RNA interference resulted in decreased cell adhesion. Mutation analyses revealed that a region of 86 amino acids located between aa 139 and aa 225 was responsible for maspin effect on adhesion. In addition, we show that maspin is associated with detergent-insoluble cortical cytoskeleton elements. Collectively, these results suggest that maspin is part of the supramolecular structure of the adhesion plaque and it modulates cell adhesion via a beta1 integrin-dependent mechanism.
Maspin是一种肿瘤抑制性丝氨酸蛋白酶抑制剂（serpin），可抑制细胞侵袭和迁移。在此，我们分析了maspin在未转化乳腺上皮细胞的细胞黏附中的功能，并研究了该过程中涉及的潜在机制。我们报告称，maspin在细胞黏附过程的早期步骤中发挥作用。添加重组maspin可迅速增加MCF-10A细胞与内源性沉积基质的黏附，相反，抗maspin抗体（Ab）和通过RNA干扰敲低maspin均导致细胞黏附减少。突变分析表明，位于第139位氨基酸至第225位氨基酸之间的86个氨基酸区域负责maspin对黏附的影响。此外，我们表明maspin与去污剂不溶性皮质细胞骨架成分相关。总的来说，这些结果表明maspin是黏附斑超分子结构的一部分，并且它通过β1整合素依赖性机制调节细胞黏附。
