[硝酸盐胁迫及恢复对黄瓜幼苗叶片叶绿素荧光参数和ATP酶活性的影响]

[Effects of NO3- stress and recovery on chlorophyll fluorescence parameters and Atpase activities of cucumber seedling leaves].

作者信息

Yang Fengjuan, Wang Xiufeng, Wei Min, Su Xiurong, Yan Tong

机构信息

College of Horticulture Science and Engineering, Shandong Agricultural Univeristy, Tai'an 271018, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2006 Mar;17(3):403-7.

PMID:16724732

Abstract

With water culture, this paper studied the effects of NO3- stress and recovery on the chlorophyll contents, chlorophyll fluorescence parameters, and ATPase activities of cucumber seedling leaves. The results showed that under stress for 7 days, the chlorophyll a, chlorophyll b, total chlorophyll and carotenoid contents, intrinsic fluorescence (Fo), and Mg-ATPase and Ca-ATPase activities were increased significantly when the NO3- concentration was high (168 mmol x L(-1)), while the intrinsic photochemical efficiency (Fv/Fm), potential activities (Fv/Fo) and quantum yield (phiPS II) of PSII were decreased with increasing NO3- concentration. After recovery for 7 days, the chlorophyll and carotenoid contents of all treatments with NO3- were lower than CK, while the intrinsic fluorescence, intrinsic photochemical efficiency and quantum yield of PS II were similar to CK when the NO3- concentration was < or = 84 mmol x L((-1), and the Fv/Fm and phiPS II were significantly lower than CK when the NO3-concentration was > 84 mmol x L(-1). The potential activity of PS II was similar to CK when the NO3- concentration was 42 and 126 mmol x L(-1), but significantly lower than CK in other treatments. The activities of Mg-ATPase and Ca-ATPase were decreased firstly but increased then when the NO3- concentration was > 84 mmol x L(-1).

摘要

采用水培法，研究了NO₃⁻胁迫及恢复处理对黄瓜幼苗叶片叶绿素含量、叶绿素荧光参数和ATP酶活性的影响。结果表明，胁迫7 d时，高NO₃⁻浓度（168 mmol·L⁻¹）下叶绿素a、叶绿素b、总叶绿素和类胡萝卜素含量、初始荧光（Fo）以及Mg-ATP酶和Ca-ATP酶活性显著增加，而PSII的初始光化学效率（Fv/Fm）、潜在活性（Fv/Fo）和量子产量（φPS II）随NO₃⁻浓度升高而降低。恢复7 d后，NO₃⁻处理的所有处理叶绿素和类胡萝卜素含量均低于对照，当NO₃⁻浓度≤84 mmol·L⁻¹时，PSII的初始荧光、初始光化学效率和量子产量与对照相似，当NO₃⁻浓度>84 mmol·L⁻¹时，Fv/Fm和φPS II显著低于对照。当NO₃⁻浓度为42和126 mmol·L⁻¹时，PSII的潜在活性与对照相似，但其他处理显著低于对照。当NO₃⁻浓度>84 mmol·L⁻¹时，Mg-ATP酶和Ca-ATP酶活性先降低后升高。