Nissen A K, Soede N M, Hyttel P, Schmidt M, D'Hoore L
Dept. of Anatomy and Physiology, Royal Veterinary and Agricultural University Bülowsvej 13, DK-1870 Frederiksberg C., Denmark.
Theriogenology. 1997 Jun;47(8):1571-82. doi: 10.1016/s0093-691x(97)00162-3.
The objective of this experiment was to identify the optimal time of insemination relative to the time of ovulation, based on ultrasonographic detection of embryonic survival at 10 days after ovulation, number of sows farrowing, and litter size. Furthermore, the possible value of the interval from weaning to onset of estrus for prediction of the time of ovulation was examined. Crossbred sows (n = 143) that had farrowed 2 to 9 litters were weaned (Day 0) and observed for estrus every 8 h from Day 3 until end of estrus. Ultrasonography was performed every 6 h, from 12 h after onset of estrus until ovulation had been observed. The sows were inseminated once at various time intervals from ovulation. At Day 16, 25 of the sows were slaughtered and their uteri were flushed for embryos. In the remaining sows, the number of viable and dead piglets and mummified fetuses per sow was recorded at farrowing, with the sum of the 3 constituting the total number of piglets born per sow. The highest number of embryos recovered per sow was found after insemination during the interval from 24 h before to 4 h after ovulation. The lowest frequency of non-pregnant sows and the highest total number of piglets born per sow were found after insemination from 28 h before to 4 h after ovulation. Consequently, the optimal time for insemination was found to be in the interval 28 h before to 4 h after ovulation. The interval from weaning to onset of estrus and from onset of estrus to ovulation were negatively correlated, allowing a rough prediction of the time of ovulation from the interval from weaning to onset of estrus.
本实验的目的是基于排卵后10天胚胎存活情况的超声检测、产仔母猪数量和窝产仔数,确定相对于排卵时间的最佳授精时间。此外,还研究了断奶至发情开始的间隔时间对预测排卵时间的可能价值。对已产2至9窝仔猪的杂交母猪(n = 143)进行断奶(第0天),从第3天开始每8小时观察一次发情情况,直至发情结束。从发情开始后12小时起,每6小时进行一次超声检查,直至观察到排卵。母猪在排卵后的不同时间间隔进行一次授精。在第16天,宰杀25头母猪,冲洗其子宫以获取胚胎。在其余母猪中,记录每头母猪产仔时存活和死亡仔猪以及木乃伊化胎儿的数量,这三者之和即为每头母猪出生仔猪的总数。每头母猪在排卵前24小时至排卵后4小时的间隔内授精后,回收的胚胎数量最多。在排卵前28小时至排卵后4小时的间隔内授精后,未怀孕母猪的频率最低,每头母猪出生仔猪的总数最高。因此,发现最佳授精时间为排卵前28小时至排卵后4小时的间隔内。断奶至发情开始的间隔时间以及发情开始至排卵的间隔时间呈负相关,据此可以根据断奶至发情开始的间隔时间大致预测排卵时间。