利用激光扫描共聚焦荧光显微镜对单殖吸虫硬体进行三维成像。
Three-dimensional imaging of monogenoidean sclerites by laser scanning confocal fluorescence microscopy.
作者信息
Galli Paolo, Strona Giovanni, Villa Anna Maria, Benzoni Francesca, Fabrizio Stefani, Doglia Silvia Maria, Kritsky Delane C
机构信息
Department of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.
出版信息
J Parasitol. 2006 Apr;92(2):395-9. doi: 10.1645/GE-3544RN.1.
A nondestructive protocol for preparing specimens of Monogenoidea for both alpha-taxonomic studies and reconstruction of 3-dimensional structure is presented. Gomori's trichrome, a stain commonly used to prepare whole-mount specimens of monogenoids for taxonomic purposes, is used to provide fluorescence of genital spines, the copulatory organ, accessory piece, squamodisc, anchors, hooks, bars, and clamps under laser scanning confocal microscopy.
本文介绍了一种用于单殖吸虫标本制备的无损方法，可用于α分类学研究和三维结构重建。戈莫里三色染色法是一种常用于制备单殖吸虫全封片标本以进行分类学研究的染色方法，该方法可使生殖棘、交配器官、辅助片、鳞盘、锚、钩、棒和夹钳在激光扫描共聚焦显微镜下发出荧光。
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