蜱传黄病毒的3'非翻译区起源于开放阅读框内长重复序列的复制。

The 3' untranslated region of tick-borne flaviviruses originated by the duplication of long repeat sequences within the open reading frame.

作者信息

Gritsun T S, Gould E A

机构信息

Centre for Ecology and Hydrology, Mansfield Rd, Oxford 0X1 3SR, UK.

出版信息

Virology. 2006 Jul 5;350(2):269-75. doi: 10.1016/j.virol.2006.03.002. Epub 2006 May 26.

DOI:10.1016/j.virol.2006.03.002

PMID:16730048

Abstract

Comparative alignment of the 3'untranslated regions (3'UTRs) of tick-borne flaviviruses has previously revealed short direct repeat sequences about 25-70 nucleotides long [Gritsun, T.S., Venugopal, K., Zanotto, P.M., Mikhailov, M.V., Sall, A.A., Holmes, E.C., Polkinghorne, I., Frolova, T.V., Pogodina, V.V., Lashkevich, V.A., Gould, E.A., 1997. Complete sequence of two tick-borne flaviviruses isolated from Siberia and the UK: analysis and significance of the 5' and 3'-UTRs. Virus Res. 49 (1) 27-39; Wallner, G., Mandl, C.W., Kunz, C., Heinz, F.X., 1995. The flavivirus 3'-noncoding region: extensive size heterogeneity independent of evolutionary relationships among strains of tick-borne encephalitis virus. Virology, 213 (1) 169-178]. We now show that these short sequences appear to have originated from longer repeat sequences (LRSs) that are present both in the 3'UTR and the open reading frame of the genome. We propose that the 3'UTR, and possibly the open reading frame, evolved through multiple duplications, deletions and mutations of a primordial sequence element.

摘要

蜱传黄病毒3'非翻译区（3'UTR）的比较比对先前已揭示出长度约为25 - 70个核苷酸的短直接重复序列[格里茨恩，T.S.，韦努戈帕尔，K.，扎诺托，P.M.，米哈伊洛夫，M.V.，萨尔，A.A.，霍姆斯，E.C.，波尔金霍恩，I.，弗罗洛娃，T.V.，波戈迪娜，V.V.，拉什克维奇，V.A.，古尔德，E.A.，1997年。从西伯利亚和英国分离的两种蜱传黄病毒的完整序列：5'和3'UTR的分析及意义。病毒研究。49(1) 27 - 39；瓦尔纳，G.，曼德尔，C.W.，昆茨，C.，海因茨，F.X.，1995年。黄病毒3'非编码区：蜱传脑炎病毒株间广泛的大小异质性与进化关系无关。病毒学，213(1) 169 - 178]。我们现在表明，这些短序列似乎起源于基因组3'UTR和开放阅读框中都存在的较长重复序列（LRSs）。我们提出，3'UTR以及可能的开放阅读框是通过原始序列元件的多次重复、缺失和突变进化而来的。